即用型ABTS溶液 适合HRP标记的ELISA检测

货号11001存储条件 在2-8度冷藏保存, 避免光照
规格1 L价格2280
Ex (nm)420Em (nm)
分子量溶剂
产品详细介绍


简要概述

即用型ABTS溶液是美国AAT Bioquest生产的ELISA检测试剂,辣根过氧化物酶(HRP)和HRP缀合物在过氧化氢存在下促进ABTS氧化,将ABTS转化为其蓝绿色氧化产物。该显色反应广泛用于ELISA测定中的HRP定量。氧化的ABTS产物具有420nm的最大吸收,可以用分光光度计容易地跟踪。ReadiUse™ABTS解决方案针对在微孔板或试管中使用HRP或HRP标记的缀合物和过氧化氢的ELISA测定进行了优化。我们的ABTS解决方案可以在一次添加中完成HRP反应。在过氧化氢存在下,当与HRP或HRP缀合物反应时,测定溶液将其颜色改变为浅绿色。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的即用型ABTS溶液。 

 

适用仪器


光吸收酶标仪  
吸收: 420nm
推荐孔板: 纯白色


产品说明书

样品实验方案

1.在HRP标记的试剂温育后洗涤测定板。

2.每孔加入100μLReadiUse ABTS溶液。

3.将板在室温下孵育15-30分钟。注意:孵育时间取决于测定条件。

4.用ELISA酶标仪测量415±10 nm(最大420 nm)的吸光度信号。注意:如果需要,可以通过向0.1M柠檬酸中加入等体积的1%SDS或0.01%叠氮化钠来终止反应。
 
 
参考文献

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Broccoli processing wastes as a source of peroxidase
Authors: Duarte-Vazquez MA, Garcia-Padilla S, Garcia-Almendarez BE, Whitaker JR, Regalado C.
Journal: J Agric Food Chem (2007): 10396

 

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说明书
即用型ABTS溶液 适合HRP标记的ELISA检测 .pdf