ReadiUse TMB底物溶液*适用于ELISA检测HRP偶联物

货号11012存储条件 在2-8度冷藏保存, 避免光照
规格100 ml价格984
Ex (nm)650Em (nm)
分子量240.34溶剂
产品详细介绍


简要概述

ReadiUse TMB底物溶液是美国AAT Bioquest生产的ReadiUse系列染料,辣根过氧化物酶(HRP)和HRP缀合物在过氧化氢存在下促进ABTS氧化,将ABTS转化为其蓝绿色氧化产物。 ReadiUse TMB底物溶液是TMB底物与过氧化氢的预混溶液。 它在与HRP或HRP缀合物相互作用时产生蓝色产物而不添加过氧化氢。 可溶性蓝色产物可在650nm下定量。 使用终止溶液可以将灵敏度提高2-4倍,并且可以在450nm处读取所得的黄色溶液。 ReadiUse TMB底物解决方案提供了一种方便且超灵敏的定量底物系统。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的ReadiUse TMB底物溶液。 

 

适用仪器


光吸收酶标仪  
吸收: 650nm
推荐孔板: 纯白色


产品说明书

样品实验方案

1.在HRP标记的试剂温育后洗涤测定板。

2.每孔加入100μLReadiUse TMB溶液。

3.将板在室温下孵育15-30分钟或直至产生所需的颜色。注意:孵育时间取决于测定条件。

4.用ELISA酶标仪测量650 nm处的吸光度信号。注意:如果需要,可以通过向每个孔中加入等体积的2M硫酸来终止反应。停止反应应在450 nm处读取。

 

参考文献

5,10,15,20-tetrakis(4-carboxyl phenyl)porphyrin-CdS nanocomposites with intrinsic peroxidase-like activity for glucose colorimetric detection
Authors: Liu Q, Jia Q, Zhu R, Shao Q, Wang D, Cui P, Ge J.
Journal: Mater Sci Eng C Mater Biol Appl (2014): 177

Au nanoparticles on citrate-functionalized graphene nanosheets with a high peroxidase-like performance
Authors: Chen X, Tian X, Su B, Huang Z, Oyama M.
Journal: Dalton Trans (2014): 7449

Biogenic magnetic nanoparticles from Burkholderia sp. YN01 exhibiting intrinsic peroxidase-like activity and their applications
Authors: Pan Y, Li N, Mu J, Zhou R, Xu Y, Cui D, Wang Y, Zhao M.
Journal: Appl Microbiol Biotechnol. (2014)

Colorimetric estimation of human glucose level using gamma-Fe(2)O(3) nanoparticles: an easily recoverable effective mimic peroxidase
Authors: Mitra K, Ghosh AB, Sarkar A, Saha N, Dutta AK.
Journal: Biochem Biophys Res Commun (2014): 30

Glucose-sensitive colorimetric sensor based on peroxidase mimics activity of porphyrin-Fe3O4 nanocomposites
Authors: Liu Q, Li H, Zhao Q, Zhu R, Yang Y, Jia Q, Bian B, Zhuo L.
Journal: Mater Sci Eng C Mater Biol Appl (2014): 142

Interactions of macromolecular crowding agents and cosolutes with small-molecule substrates: effect on horseradish peroxidase activity with two different substrates
Authors: Aumiller WM, Jr., Davis BW, Hatzakis E, Keating CD.
Journal: J Phys Chem B (2014): 10624

NiO nanoparticles modified with 5,10,15,20-tetrakis(4-carboxyl pheyl)-porphyrin: Promising peroxidase mimetics for HO and glucose detection
Authors: Liu Q, Yang Y, Li H, Zhu R, Shao Q, Yang S, Xu J.
Journal: Biosens Bioelectron (2014): 147

Novel tungsten carbide nanorods: an intrinsic peroxidase mimetic with high activity and stability in aqueous and organic solvents
Authors: Li N, Yan Y, Xia BY, Wang JY, Wang X.
Journal: Biosens Bioelectron (2014): 521

Peroxidase-like activity of apoferritin paired gold clusters for glucose detection
Authors: Jiang X, Sun C, Guo Y, Nie G, Xu L.
Journal: Biosens Bioelectron (2014): 165

Peroxidase-Like Catalytic Activity of Ag3PO4 Nanocrystals Prepared by a Colloidal Route
Authors: Liu Y, Zhu G, Yang J, Yuan A, Shen X.
Journal: PLoS One (2014): e109158

 

相关产品

产品名称 货号
即用型ABTS溶液 适合HRP标记的ELISA检测 Cat#11001


说明书
ReadiUse TMB底物溶液*适用于ELISA检测HRP偶联物 .pdf