预活化HRP NHS酯

货号11025存储条件 在零下15度以下保存
规格300 ug价格1236
Ex (nm)Em (nm)
分子量溶剂
产品详细介绍


简要概述

ReadiUse 预活化的HRP NHS酯是HRP的单NHS酯。 它可用于容易地标记蛋白质(例如抗体)或具有氨基的其他生物分子。 ReadiUse 预活化HRP NHS酯易于使用。 它可以用于通过简单混合标记抗体,即抗体的碱性溶液(pH 8.5-9.0)可以直接与HRP NHS酯混合,并将反应混合物摇动1-2小时。 在大多数情况下,所得溶液可直接用于ELISA测定而无需进一步纯化。 ReadiUse 预活化的HRP NHS酯提供了最强大的方法来执行抗体与HRP的缀合。 任何合适量的抗体(> 1mg / mL)可以容易地与预活化的HRP NHS酯缀合而无需纯化。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的预活化HRP NHS酯。 



产品说明书

样品实验方案

1.抗体(IgG)~100μg在20μL~50μL的25mM碳酸氢钠缓冲液(pH = 8.5)中。

2.将IgG溶液转移到HRP-SE小瓶中,充分混合。

3.在室温下反应1~2小时,优选在反应过程中轻轻摇动。

4.加入等体积的100mM Tris-HCl缓冲液(pH = 7.5)+ 0.5%BSA。

5.将混合物储存在4oC,即可使用。 注意:如果目标蛋白质溶液含有其他蛋白质(如BSA),则纯化蛋白质溶液或干扰标记反应。 注意:需要除去叠氮化钠等小分子。 注意: Tris缓冲液和其他胺类化合物也会干扰标记反应。

 

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Patterned Photonic Nitrocellulose for Pseudo-Paper ELISA
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Spinal Cord Inflammation: Molecular Imaging after Thoracic Aortic Ischemia Reperfusion Injury
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Myeloperoxidase Nuclear Imaging for Epileptogenesis
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Myeloperoxidase--Hepatocyte--Stellate Cell Cross Talk Promotes Hepatocyte Injury and Fibrosis in Experimental Nonalcoholic Steatohepatitis
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Ordered cleavage of myeloperoxidase ester bonds releases active site heme leading to inactivation of myeloperoxidase by benzoic acid hydrazide analogs
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说明书
预活化HRP NHS酯 .pdf