iFluor 488酪胺
货号11060存储条件F/L
规格1 mg价格0
Ex (nm)491Em (nm)514
分子量531.47溶剂
产品详细介绍


简要概述

产品基本信息

货号:11060

产品名称:iFluor 488酪酰胺

规格:1mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:980.67

溶剂:DMSO

激发波长(nm):495

发射波长(nm):518

 

产品介绍

        iFluor 488酪胺是美国AAT Bioquest生产的用于荧光标记的试剂,对于许多免疫组织化学(IHC)应用,传统的酶促扩增程序足以实现足够的抗原检测。但是,有几个因素限制了这些程序的敏感性和实用性。 Tyramide信号放大(TSA)已被证明是一种特别通用且强大的酶扩增技术,提高了检测灵敏度。TSA依靠HRP在低浓度过氧化氢的存在下将含有标记酪胺的底物转化为氧化的高反应性自由基的能力,该自由基可与HRP处或附近的酪氨酸残基共价结合。为了实现最大IHC检测,酪胺用荧光团预标记。每个过氧化物酶标记物的多个酪胺底物转换所赋予的信号放大转化了对低丰度靶标的超灵敏检测以及使用较少量的抗体和杂交探针。在免疫组织化学应用中,源自TSA方法的灵敏度增强允许增加一级抗体稀释以减少非特异性背景信号,并且可以克服由次优固定程序或低水平靶表达引起的弱免疫标记。 iFluor™488酪胺含有极其明亮且可光稳定的iFluor™488,可通过标准FITC过滤器试剂盒轻松检测到。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的iFluor 488酪胺检测试剂。 

 

参考文献

Enhanced detection of Porcine reproductive and respiratory syndrome virus in fixed tissues by in situ hybridization following tyramide signal amplification
Authors: Trang NT, Hirai T, Ngan PH, Lan NT, Fuke N, Toyama K, Yamamoto T, Yamaguchi R.
Journal: J Vet Diagn Invest (2015): 326

Tyramide Signal Amplification for Immunofluorescent Enhancement
Authors: Faget L, Hnasko TS.
Journal: Methods Mol Biol (2015): 161

KSHV cell attachment sites revealed by ultra sensitive tyramide signal amplification (TSA) localize to membrane microdomains that are up-regulated on mitotic cells
Authors: Garrigues HJ, Rubinchikova YE, Rose TM.
Journal: Virology (2014): 75

Multiplexed immunohistochemistry, imaging, and quantitation: a review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis
Authors: Stack EC, Wang C, Roman KA, Hoyt CC.
Journal: Methods (2014): 46

Rapid and sensitive detection of Escherichia coli O157:H7 in milk and ground beef using magnetic bead-based immunoassay coupled with tyramide signal amplification
Authors: Aydin M, Herzig GP, Jeong KC, Dunigan S, Shah P, Ahn S.
Journal: J Food Prot (2014): 100

Sensitive whole-mount fluorescent in situ hybridization in zebrafish using enhanced tyramide signal amplification
Authors: Lauter G, Soll I, Hauptmann G.
Journal: Methods Mol Biol (2014): 175

Characterization of GABAergic neurons in the mouse lateral septum: a double fluorescence in situ hybridization and immunohistochemical study using tyramide signal amplification
Authors: Zhao C, Eisinger B, Gammie SC.
Journal: PLoS One (2013): e73750

Quantification of alpha-tubulin isotypes by sandwich ELISA with signal amplification through biotinyl-tyramide or immuno-PCR
Authors: Draberova E, Stegurova L, Sulimenko V, Hajkova Z, Draber P.
Journal: J Immunol Methods (2013): 63

Development of a near-infrared fluorescence ELISA method using tyramide signal amplification
Authors: Gong H, Cradduck M, Cheung L, Olive DM.
Journal: Anal Biochem (2012): 27

Integrated tyramide and polymerization-assisted signal amplification for a highly-sensitive immunoassay
Authors: Yuan L, Xu L, Liu S.
Journal: Anal Chem (2012): 10737



说明书
iFluor 488酪胺.pdf