磷酸基团绿色荧光底物 PhosLite

货号11630存储条件 在零下15度以下保存, 避免光照
规格1 mg价格2544
Ex (nm)345Em (nm)520
分子量589.49溶剂Water
产品详细介绍


简要概述

产品基本信息

货号:11630

产品名称:磷酸基团绿色荧光底物 PhosLite 

规格:1mg

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:589.49

溶剂:水

激发波长(nm):345

发射波长(nm):520

 

产品介绍

磷酸基团绿色荧光底物 PhosLite 是美国AAT Bioquest生产的用于磷酸的试剂盒,这种水溶性PhosLite 绿色基质在磷酸酶活性位点产生明亮且光稳定的黄绿色荧光沉淀。其荧光沉淀具有几种独特的性质(例如极大的斯托克斯位移和高光稳定性),使该底物成为5-溴-4-氯-3-吲哚基磷酸酯(BCIP)的优异荧光形式,BCIP是用于敏感比色检测碱性磷酸酶活性。它可用于免疫印迹,原位杂交和免疫组织化学,但不需要加入硝基蓝四唑氯化物(NBT)。碱性磷酸酶通常与二抗结合。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的磷酸基团绿色荧光底物 PhosLite 。 

 

参考文献

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A novel fluorescent transcriptional reporter for cell-based microarray assays
Authors: Redmond TM, Uhler MD.
Journal: Methods Mol Biol (2011): 41

Detection of extracellular phosphatase activity at the single-cell level by enzyme-labeled fluorescence and flow cytometry: the importance of time kinetics in ELFA labeling
Authors: Duhamel S, Gregori G, Van Wambeke F, Nedoma J.
Journal: Cytometry A (2009): 163

Modulation of cultured neural networks using neurotrophin release from hydrogel-coated microelectrode arrays
Authors: Jun SB, Hynd MR, Dowell-Mesfin NM, Al-Kofahi Y, Roysam B, Shain W, Kim SJ.
Journal: J Neural Eng (2008): 203

Duodenal brush border intestinal alkaline phosphatase activity affects bicarbonate secretion in rats
Authors: Akiba Y, Mizumori M, Guth PH, Engel E, Kaunitz JD.
Journal: Am J Physiol Gastrointest Liver Physiol (2007): G1223

Fluid shear stress induces less calcium response in a single primary osteocyte than in a single osteoblast: implication of different focal adhesion formation
Authors: Kamioka H, Sugawara Y, Murshid SA, Ishihara Y, Honjo T, Takano-Yamamoto T.
Journal: J Bone Miner Res (2006): 1012

Detecting the phosphate status of phytoplankton by enzyme-labelled fluorescence and flow cytometry
Authors: Dignum M, Hoogveld HL, Matthijs HC, Laanbroek HJ, Pel R.
Journal: FEMS Microbiol Ecol (2004): 29

Enzymatic activity of alkaline phosphatase inside protein and polymer structures fabricated via multiphoton excitation
Authors: Basu S, Campagnola PJ.
Journal: Biomacromolecules (2004): 572

Analysis of violet-excited fluorochromes by flow cytometry using a violet laser diode
Authors: Telford WG, Hawley TS, Hawley RG.
Journal: Cytometry A (2003): 48

Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics
Authors: Nedoma J, Strojsova A, Vrba J, Komarkova J, Simek K.
Journal: Environ Microbiol (2003): 462



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