Cell Meter 胞内NADH / NADPH荧光成像分析试剂盒
货号15290存储条件F/L
规格100 Tests价格5940
Ex (nm)540Em (nm)590
分子量溶剂
产品详细介绍


简要概述

        Cell Meter 胞内NADH / NADPH荧光成像分析试剂盒是美国AAT Bioquest生产的用于检测NADH/NADPH的试剂盒,细胞内二氢烟酰胺腺嘌呤二核苷酸NADH及其磷酸酯NADPH的检测对于疾病诊断和药物发现是重要的。通常,氧化还原偶联NAD / NADH和NADP / NADPH在能量代谢,糖酵解,三羧酸循环和线粒体呼吸中起关键作用。细胞中NAD(P)H水平的增加与细胞内的相关的活性氧(ROS)的异常产生和DNA损伤。然而,由于缺乏敏感的NAD(P)H探针,在生物系统中检测细胞内NAD(P)H具有挑战性。 Cell Meter™细胞内NADH / NADPH荧光成像试剂盒提供了监测活细胞中细胞内NAD(P)H水平的有效方法。 JZL1707 NAD(P)H传感器是一种优秀的荧光探针,用于检测和成像细胞中的NADH / NADPH。探针结合NADH / NADPH以产生具有高灵敏度和特异性的强荧光信号。 JZL1707 NAD(P)H传感器可以很容易地加载到活细胞中,使用Cy3®TRITC过滤器可以方便地监测其荧光信号。该试剂盒针对荧光成像和酶标仪应用进行了优化。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的Cell Meter 胞内NADH / NADPH荧光成像分析试剂盒。 

点击查看光谱



产品说明书

实验示例

概述

1.在生长培养基中制备细胞

2.将细胞与测试化合物和JZL1707 NAD(P)H传感器工作溶液在37oC孵育30-60分钟

3.将细胞洗净并保持在测定缓冲液中

4.在Ex / Em = 540/590 nm(截止波长= 570 nm)或使用TRITC滤光片的荧光显微镜下监测荧光强度(底部读取模式)

 

操作步骤

1.刺激NADP / NADPH,用10μL10X试验化合物(96孔板)或5μL5X试验化合物(384孔板)在无血清培养基或所需缓冲液(如PBS或HHBS)中处理细胞)。 对于对照孔(未处理的细胞),加入相应量的培养基或化合物缓冲液。

注意:JZL1707 NAD(P)H传感器对血清敏感,因此建议将细胞保存在无血清培养基或您选择的缓冲液中。或者,可以在常规的全培养基中制备和处理细胞。 与JZL1707 NAD(P)H传感器一起孵育时,更换为您选择的无血清培养基或缓冲液。

2.在细胞板中加入100μL/孔(96孔板)或25μL/孔(384孔板)的JZL1707 NAD(P)H传感器工作溶液。 将细胞与测试化合物和JZL1707 NAD(P)H传感器工作溶液在37oC共孵育30-60分钟,避光。

注意:对于NADH / NADPH阳性对照处理:将HeLa细胞与100μMNADH或NADPH在无血清培养基中孵育30分钟,并与JZL1707 NAD(P)H传感器工作溶液在37℃下共孵育30分钟。 详细信息请参见图1。

3.用所需缓冲液洗涤细胞一次。 移除每个孔中的溶液,并添加100μL/孔的测定缓冲液(组分B)用于96孔板或25μL/孔用于384孔板。

4.使用具有底部读取模式的Ex / Em = 540 / 590nm(截止= 570nm)的酶标仪监测荧光增加,或使用荧光显微镜和Cy3过滤器或TRITC过滤器组拍摄图像。

 

参考文献

Celastrol attenuates angiotensin II mediated human umbilical vein endothelial cells damage through activation of Nrf2/ERK1/2/Nox2 signal pathway
Authors: Miao Li, Xin Liu, Yongpeng He, Qingyin Zheng, Min Wang, Yu Wu, Yuanpeng Zhang, Chaoyun Wang
Journal: European Journal of Pharmacology (2017): 124--133

Cytosolic Redox Status of Wine Yeast (Saccharomyces Cerevisiae) under Hyperosmotic Stress during Icewine Fermentation
Authors: Fei Yang, Caitlin Heit, Debra L Inglis
Journal: Fermentation (2017): 61

Epigenetic regulation of Runx2 transcription and osteoblast differentiation by nicotinamide phosphoribosyltransferase
Authors: Min Ling, Peixin Huang, Shamima Islam, Daniel P Heruth, Xuanan Li, Li Qin Zhang, Ding-You Li, Zhaohui Hu, Shui Qing Ye
Journal: Cell & Bioscience (2017): 27

MCU-dependent mitochondrial Ca2+ inhibits NAD+/SIRT3/SOD2 pathway to promote ROS production and metastasis of HCC cells
Authors: T Ren, H Zhang, J Wang, J Zhu, M Jin, Y Wu, X Guo, L Ji, Q Huang, H Yang
Journal: Oncogene (2017)

Metabolic and molecular insights into an essential role of nicotinamide phosphoribosyltransferase
Authors: Li Q Zhang, Leon Van Haandel, Min Xiong, Peixin Huang, Daniel P Heruth, Charlie Bi, Roger Gaedigk, Xun Jiang, Ding-You Li, Gerald Wyckoff
Journal: Cell Death & Disease (2017): e2705

Pyrroloquinoline Quinone, a Redox-active o-Quinone, Stimulates Mitochondrial Biogenesis by Activating SIRT1/PGC-1α Signaling Pathway
Authors: Kazuhiro Saihara, Ryosuke Kamikubo, Kazuto Ikemoto, Koji Uchida, Mitsugu Akagawa
Journal: Biochemistry (2017)

Resveratrol attenuates excessive ethanol exposure induced insulin resistance in rats via improving NAD+/NADH ratio
Authors: Gang Luo, Bingqing Huang, Xiang Qiu, Lin Xiao, Ning Wang, Qin Gao, Wei Yang, Liping Hao
Journal: Molecular Nutrition & Food Research (2017)

A Snapshot of the Plant Glycated Proteome STRUCTURAL, FUNCTIONAL, AND MECHANISTIC ASPECTS
Authors: Tatiana Bilova, Elena Lukasheva, Dominic Brauch, Uta Greifenhagen, Gagan Paudel, Elena Tarakhovskaya, Nadezhda Frolova, Juliane Mittasch, Gerd Ulrich Balcke, Alain Tissier
Journal: Journal of Biological Chemistry (2016): 7621--7636

AMPK activation protects cells from oxidative stress-induced senescence via autophagic flux restoration and intracellular NAD+ elevation
Authors: Xiaojuan Han, Haoran Tai, Xiaobo Wang, Zhe Wang, Jiao Zhou, Xiawei Wei, Yi Ding, Hui Gong, Chunfen Mo, Jie Zhang
Journal: Aging cell (2016): 416--427

Cell-Line Selectivity Improves the Predictive Power of Pharmacogenomic Analyses and Helps Identify NADPH as Biomarker for Ferroptosis Sensitivity
Authors: Kenichi Shimada, Miki Hayano, Nen C Pagano, Brent R Stockwell
Journal: Cell chemical biology (2016): 225--235

 

相关产品

产品名称 货号
Cell Meter 细胞内NADH / NADPH荧光成像试剂盒 深红色荧光 Cat#15295


说明书
Cell Meter 胞内NADH / NADPH荧光成像分析试剂盒.pdf