Cell Meter 胞内NADH / NADPH流式细胞分析试剂盒
货号15291存储条件F/L
规格100 Tests价格5940
Ex (nm)540Em (nm)590
分子量溶剂
产品详细介绍


简要概述

        Cell Meter 胞内NADH / NADPH流式细胞分析试剂盒是美国AAT Bioquest生产的用于检测NADH/NADPH的试剂盒,细胞内二氢烟酰胺腺嘌呤二核苷酸NADH及其磷酸酯NADPH的检测对于疾病诊断和药物发现是重要的。通常,氧化还原偶联NAD / NADH和NADP / NADPH在能量代谢,糖酵解,三羧酸循环和线粒体呼吸中起关键作用。细胞中NAD(P)H水平的增加与活性氧(ROS)和DNA损伤的异常产生有关。然而,由于缺乏敏感的NAD(P)H探针,在生物系统中检测细胞内NAD(P)H具有挑战性。 Cell Meter 细胞内NADH / NADPH流式细胞分析试剂盒提供了一种监测活细胞内细胞内NAD(P)H水平的有效方法。 JZL1707 NAD(P)H传感器是一种优秀的荧光探针,用于检测和成像细胞中的NADH / NADPH。探针结合NADH / NADPH以产生具有高灵敏度和特异性的强荧光信号。 JZL1707 NAD(P)H传感器可以很容易地加载到活细胞中,并且可以使用PE通道中的流式细胞仪方便地监测其荧光信号。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的Cell Meter 胞内NADH / NADPH流式细胞分析试剂盒。 

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产品说明书

实验示例

概述

1.准备细胞(0.5-1×106细胞/ mL)
2.将细胞与测试化合物和JZL1707 NAD(P)H传感器在37ºC下孵育30-60分钟
3.洗涤细胞并将其保存在测定缓冲液中
4.使用PE通道通过流式细胞仪分析细胞

 

操作步骤

1.对于每个样品,在0.5 mL无血清培养基或您选择的缓冲液中以1×105至1×106细胞/ mL的密度制备细胞。注意:应单独评估每个细胞系,以确定最佳细胞密度。对于粘附细胞,用0.5 mM EDTA轻轻提起细胞以保持细胞完整,并用无血清培养基洗涤细胞一次。注意:JZL1707 NAD(P)H传感器对血清敏感,因此建议将细胞保存在您选择的无血清培养基或缓冲液中。或者,可以制备细胞并在常规完全培养基中处理。与JZL1707 NAD(P)H Sensor孵育时,请更改为无血清培养基或您选择的缓冲液。
在37ºC下将细胞与测试化合物一起孵育所需的时间,以刺激细胞内的NADH / NADPH。注意:适当的孵育时间取决于所用的单个细胞类型和测试化合物。优化每个实验的孵育时间。

2.将1 µL JZL1707 NAD(P)H传感器(组分A)加入0.5 mL细胞悬液中。在37ºC下孵育30-60分钟。注意:对于NADH / NADPH阳性对照处理:将Jurkat细胞与100 µM NADH或NADPH在无血清培养基中孵育30分钟,然后与JZL1707 NAD(P)H Sensor工作溶液在37ºC共同孵育30分钟。分钟。有关详细信息,请参见图1。

3.用所需的缓冲液洗涤细胞一次。将细胞保存在测定缓冲液(组分B)中。

4.使用流式细胞仪监测PE通道的荧光强度。

 

参考文献

Celastrol attenuates angiotensin II mediated human umbilical vein endothelial cells damage through activation of Nrf2/ERK1/2/Nox2 signal pathway
Authors: Miao Li, Xin Liu, Yongpeng He, Qingyin Zheng, Min Wang, Yu Wu, Yuanpeng Zhang, Chaoyun Wang
Journal: European Journal of Pharmacology (2017): 124--133

Cytosolic Redox Status of Wine Yeast (Saccharomyces Cerevisiae) under Hyperosmotic Stress during Icewine Fermentation
Authors: Fei Yang, Caitlin Heit, Debra L Inglis
Journal: Fermentation (2017): 61

Epigenetic regulation of Runx2 transcription and osteoblast differentiation by nicotinamide phosphoribosyltransferase
Authors: Min Ling, Peixin Huang, Shamima Islam, Daniel P Heruth, Xuanan Li, Li Qin Zhang, Ding-You Li, Zhaohui Hu, Shui Qing Ye
Journal: Cell & Bioscience (2017): 27

MCU-dependent mitochondrial Ca2+ inhibits NAD+/SIRT3/SOD2 pathway to promote ROS production and metastasis of HCC cells
Authors: T Ren, H Zhang, J Wang, J Zhu, M Jin, Y Wu, X Guo, L Ji, Q Huang, H Yang
Journal: Oncogene (2017)

Metabolic and molecular insights into an essential role of nicotinamide phosphoribosyltransferase
Authors: Li Q Zhang, Leon Van Haandel, Min Xiong, Peixin Huang, Daniel P Heruth, Charlie Bi, Roger Gaedigk, Xun Jiang, Ding-You Li, Gerald Wyckoff
Journal: Cell Death & Disease (2017): e2705

Pyrroloquinoline Quinone, a Redox-active o-Quinone, Stimulates Mitochondrial Biogenesis by Activating SIRT1/PGC-1α Signaling Pathway
Authors: Kazuhiro Saihara, Ryosuke Kamikubo, Kazuto Ikemoto, Koji Uchida, Mitsugu Akagawa
Journal: Biochemistry (2017)

Resveratrol attenuates excessive ethanol exposure induced insulin resistance in rats via improving NAD+/NADH ratio
Authors: Gang Luo, Bingqing Huang, Xiang Qiu, Lin Xiao, Ning Wang, Qin Gao, Wei Yang, Liping Hao
Journal: Molecular Nutrition & Food Research (2017)

A Snapshot of the Plant Glycated Proteome STRUCTURAL, FUNCTIONAL, AND MECHANISTIC ASPECTS
Authors: Tatiana Bilova, Elena Lukasheva, Dominic Brauch, Uta Greifenhagen, Gagan Paudel, Elena Tarakhovskaya, Nadezhda Frolova, Juliane Mittasch, Gerd Ulrich Balcke, Alain Tissier
Journal: Journal of Biological Chemistry (2016): 7621--7636

AMPK activation protects cells from oxidative stress-induced senescence via autophagic flux restoration and intracellular NAD+ elevation
Authors: Xiaojuan Han, Haoran Tai, Xiaobo Wang, Zhe Wang, Jiao Zhou, Xiawei Wei, Yi Ding, Hui Gong, Chunfen Mo, Jie Zhang
Journal: Aging cell (2016): 416--427

Cell-Line Selectivity Improves the Predictive Power of Pharmacogenomic Analyses and Helps Identify NADPH as Biomarker for Ferroptosis Sensitivity
Authors: Kenichi Shimada, Miki Hayano, Nen C Pagano, Brent R Stockwell
Journal: Cell chemical biology (2016): 225--235

 

参考文献

产品名称 货号
Cell Meter 细胞内NADH / NADPH流式细胞分析试剂盒 深红色荧光 Cat#15296


说明书
Cell Meter 胞内NADH / NADPH流式细胞分析试剂盒.pdf