1mM Biotin-16-dUTP溶液
货号17017存储条件F/L
规格25 nmoles价格1140
Ex (nm)Em (nm)
分子量1013.72溶剂Water
产品详细介绍


简要概述

产品基本信息

货号:17017

产品名称:1mM 生物素-16-dUTP溶液

规格:25nmolse

储存条件:保存在冰箱-15℃干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:1013.72

外观:液体

溶剂:水

激发波长(nm):N/A

发射波长(nm):N/A

 

产品介绍

        生物素修饰的脱氧尿苷5'-三磷酸广泛用于各种非放射性DNA标记反应,包括切口平移,随机引物标记,cDNA标记和3'末端标记。已显示生物素化的探针以与非生物素化探针相同的速率和程度与同源核酸杂交。杂交的生物素化DNA探针可以通过抗生物素蛋白和链霉抗生物素蛋白检测。生物素-16-dUTP可以通过切口平移,随机引发,3'-末端标记或在PCR过程中酶促掺入DNA中。数字'16'是dUTP和生物素之间的接头主链中的碳原子数。接头越长,生物素与抗生物素蛋白的相互作用就越有效。另一方面,接头越短,dUTP更有效地掺入DNA。生物素-16-dUTP用于在各种杂交应用中生产生物素化的DNA探针,包括Southern印迹,Northern印迹,斑点印迹,固定细胞和组织。

 

参考文献

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A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
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说明书
1mM Biotin-16-dUTP溶液.pdf