1mM Fluorescein-dUTP溶液
货号17022存储条件F/L
规格25 nmoles价格1140
Ex (nm)492Em (nm)515
分子量1060.62溶剂Water
产品详细介绍


简要概述

产品基本信息

货号:17022

产品名称:1mM 荧光素-dUTP溶液

规格:25nmolse

储存条件:-80℃避光干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:1060.62

外观:液体

溶剂:10mM Tris-HCl + 1mM EDTA (pH=7.5)

激发波长(nm):492

发射波长(nm):515

 

产品介绍

        染料修饰的脱氧尿苷5'-三磷酸(例如氨基烯丙基-dUTP)可用于通过常规酶掺入方法如逆转录,切口平移,随机引物标记或PCR产生含染料的DNA。该酶荧光标记方法广泛用于FISH探针和基于微阵列的实验。该荧光素-dUTP缀合物可用作具有Spectrum Green™过滤器组的绿色荧光颜色(Spectrum Green ™是Vysis的商标)。

点击查看光谱

 

参考文献

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1
Authors: Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.
Journal: Biosens Bioelectron (2004): 685

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Authors: Cox WG, Singer VL.
Journal: Biotechniques (2004): 114

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX
Authors: Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.
Journal: Bioconjug Chem (2003): 919

Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
Authors: Wooddell CI, Burgess RR.
Journal: Biochemistry (2000): 13405

Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
Authors: Duplaa C, Couffinhal T, Labat L, Moreau C, Lamaziere JM, Bonnet J.
Journal: Anal Biochem (1993): 229

A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145

Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166

Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2'-deoxyuridine-5'-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953



说明书
1mM Fluorescein-dUTP溶液.pdf