1mM Tetramethylrhodamine-dUTP溶液
货号17023存储条件F/L
规格25 nmoles价格1140
Ex (nm)546Em (nm)564
分子量1115.77溶剂Water
产品详细介绍


简要概述

产品基本信息

货号:17023

产品名称:1mM 四甲基罗丹明-dUTP溶液

规格:25nmolse

储存条件:-15℃避光干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:1115.77

溶剂:水

激发波长(nm):546

发射波长(nm):564

 

产品介绍

        染料修饰的脱氧尿苷5'-三磷酸(例如氨基烯丙基dUTP)可用于通过常规酶促掺入方法(例如逆转录,切口平移,随机引物标记或PCR)产生含染料的DNA。该酶荧光标记方法广泛用于FISH探针和基于微阵列的实验。该TMR-dUTP缀合物可用作具有Spectrum Orange®过滤器组的橙色荧光颜色(Spectrum Orange®是Vysis的商标)。

点击查看光谱

 

参考文献

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Simple method for preparation of fluor/hapten-labeled dUTP
Authors: Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.
Journal: Biotechniques (2000): 518

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
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Journal: Biochemistry (2000): 13405

Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
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Journal: Anal Biochem (1993): 229

A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
Authors: Urabe T, Sano K, Tanno M, Mizoguchi J, Otani M, Lee MH, Takasaki T, Kusakabe H, Imagawa DT, Nakai M.
Journal: J Virol Methods (1992): 145

Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166

Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2'-deoxyuridine-5'-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953



说明书
1mM Tetramethylrhodamine-dUTP溶液.pdf