DAPI(4',6-二脒基-2-苯基吲哚二盐酸盐)*10mM水溶液*

货号17507存储条件f/l
规格2 mL价格948
Ex (nm)359Em (nm)457
分子量350.25溶剂Water
产品详细介绍


简要概述

产品基本信息

货号:17507

产品名称:DAPI[4,6-二脒基-2-苯基吲哚,二盐酸盐]* 10 mM水溶液*

规格:2ml

储存条件:保存在冰箱-20℃干燥

保质期:6个月

 

产品物理化学光谱特性

分子量:350.25

溶剂:水

Ex(nm):358

Em(nm):461

 

产品介绍

        DAPI是一种与DNA结合强烈的荧光染色剂。它广泛用于荧光显微镜。由于DAPI通过完整的细胞膜,它可用于除固定细胞外的活细胞染色。对于荧光显微镜,DAPI被紫外线激发。当与双链DNA结合时,其最大吸收在358nm,其发射最大值在461nm。DAPI的一个缺点是其排放相当广泛。DAPI也与RNA结合,但是它不像它与DNA结合时那样强烈地发荧光。 当与RNA结合时,其发射偏移至约500nm .DAPI的蓝色发射便于多重测定,因为DAPI与荧光素和绿色荧光蛋白(GFP)等绿色荧光分子或红色荧光染料之间的荧光重叠非常少。除了标记细胞核外,DAPI还用于检测细胞培养物中的支原体或病毒DNA。

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参考文献

Astrocytic expression of the CXCL12 receptor, CXCR7/ACKR3 is a hallmark of the diseased, but not developing CNS
Authors: Malte Puchert, Fabian Pelkner, Gregor Stein, Doychin N Angelov, Johannes Boltze, Daniel-Christoph Wagner, Francesca Odoardi, Alexander Flügel, Wolfgang J Streit, Jürgen Engele
Journal: Molecular and Cellular Neuroscience (2017)

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Fuqiang Guo, Jiajia Shang, Hai Zhao, Kangrong Lai, Yang Li, Zhongxiong Fan, Zhenqing Hou, Guanghao Su
Journal: Colloids and Surfaces B: Biointerfaces (2017)

Legionella pneumophila infection activates bystander cells differentially by bacterial and host cell vesicles
Authors: Anna Lena Jung, Christina Elena Herkt, Christine Schulz, Kathrin Bolte, Kerstin Seidel, Nicoletta Scheller, Alexandra Sittka-Stark, Wilhelm Bertrams, Bernd Schmeck
Journal: Scientific Reports (2017)

Peptides mimicking viral proteins of porcine circovirus type 2 were profiled by the spectrum of mouse anti-PCV2 antibodies
Authors: Ling-Chu Hung, Cheng-Yao Yang, Ivan-Chen Cheng
Journal: BMC immunology (2017): 25

Poly (ADP-ribose) polymerase inhibitors activate the p53 signaling pathway in neural stem/progenitor cells
Authors: Akiko Okuda, Suguru Kurokawa, Masanori Takehashi, Aika Maeda, Katsuya Fukuda, Yukari Kubo, Hyuma Nogusa, Tomoka Takatani-Nakase, Shujiro Okuda, Kunihiro Ueda
Journal: BMC neuroscience (2017): 14

Apoptotic effect of the selective PPARβ/δ agonist GW501516 in invasive bladder cancer cells
Authors: Adeline Péchery, Sylvie Fauconnet, Hugues Bittard, Isabelle Lascombe
Journal: Tumor Biology (2016): 1--14

Carbon nanotube--Protamine hybrid: Evaluation of DNA cell penetration
Authors: Cécile Caoduro, Raoudha Kacem, Khaoula Boukari, Fabien Picaud, Claire-Hélène Brachais, David Monchaud, Christophe Borg, Hatem Boulahdour, Tijani Gharbi, Régis Delage-Mourroux
Journal: Carbon (2016): 742--752

Identification of epigenetic factors regulating the mesenchyme to epithelium transition by RNA interference screening in breast cancer cells
Authors: Jean-Marc Gregoire, Laurence Fleury, Clara Salazar-Cardozo, Frédéric Alby, Véronique Masson, Paola Barbara Arimondo, Frédéric Ausseil
Journal: BMC cancer (2016): 700

Legionella pneumophila-Derived Outer Membrane Vesicles Promote Bacterial Replication in Macrophages
Authors: Anna Lena Jung, Cornelia Stoiber, Christina E Herkt, Christine Schulz, Wilhelm Bertrams, Bernd Schmeck
Journal: PLoS Pathog (2016): e1005592

Redox-Sensitive Citronellol--Cabazitaxel Conjugate: Maintained in Vitro Cytotoxicity and Self-Assembled as Multifunctional Nanomedicine
Authors: Peng Xue, Dan Liu, Jing Wang, Na Zhang, Jiahua Zhou, Lin Li, Weiling Guo, Mengchi Sun, Xiangfei Han, Yongjun Wang
Journal: Bioconjugate chemistry (2016): 1360--1372

 

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产品名称 货号
DAPI(4',6-二脒基-2-苯基吲哚二盐酸盐)CAS 28718-90-3 Cat#17510


说明书
DAPI(4',6-二脒基-2-苯基吲哚二盐酸盐)*10mM水溶液*.pdf