DAPI（4',6-二脒基-2-苯基吲哚二盐酸盐）*10mM水溶液* CAS 28718-90-3
英文名称：DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *10 mM solution in water*
产品参数
| Ex (nm) | 359 | Em (nm) | 457 |
| 分子量 | 350.25 | 溶剂 | Water |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品概述
产品货期
咨询
产品优势
1.多色检测兼容性：蓝色荧光与FITC/GFP等绿色荧光标记及Texas Red等红色染料几乎无光谱重叠
2.污染检测功能：除细胞核标记外，还可用于细胞培养中支原体或病毒DNA的检测
适用范围
用于标记活细胞与固定细胞
产品介绍
DAPI是一种广泛应用于荧光显微镜研究的DNA特异性荧光染料，其独特性质包括：
（1）具有穿透完整细胞膜的能力，既适用于固定细胞也适用于活细胞染色；
（2）紫外光激发时，与双链DNA结合后呈现358nm吸收峰和461nm蓝色发射峰；
（3）虽能与RNA结合（发射峰红移至500nm附近），但荧光强度显著弱于DNA结合状态。
该染料的优势在于其蓝色荧光与FITC/GFP等绿色荧光标记及Texas Red等红色染料几乎不发生光谱重叠，因此是多色荧光检测实验的理想选择。除常规细胞核染色外，DAPI还可用于检测细胞培养物中的支原体或病毒DNA污染。需注意的是，其较宽的发射光谱可能影响检测特异性。
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