DAPI乳酸 CAS 28718-91-4

货号17509存储条件 在零下15度以下保存, 避免光照
规格25 mg价格1272
Ex (nm)359Em (nm)457
分子量457.48溶剂Water
产品详细介绍


简要概述

产品基本信息

货号:17509

产品名称:DAPI乳酸

CAS:28718-91-4

规格:25mg

储存条件:保存在冰箱-20℃干燥

保质期:6个月

 

产品物理化学光谱特性

分子量:457.48

溶剂:水

Ex(nm):358

Em(nm):461

 

产品介绍

DAPI是一种与DNA结合强烈的荧光染色剂。它广泛用于荧光显微镜。由于DAPI通过完整的细胞膜,它可用于除固定细胞外的活细胞染色。对于荧光显微镜,DAPI被紫外线激发。当与双链DNA结合时,其最大吸收在358nm,其发射最大值在461nm。DAPI的一个缺点是其排放相当广泛。DAPI也与RNA结合,但是它不像它与DNA结合时那样强烈地发荧光。 当与RNA结合时,其发射偏移至约500nm .DAPI的蓝色发射便于多重测定,因为DAPI与荧光素和绿色荧光蛋白(GFP)等绿色荧光分子或红色荧光染料之间的荧光重叠非常少。除了标记细胞核外,DAPI还用于检测细胞培养物中的支原体或病毒DNA。

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参考文献

A low dose of ethidium bromide leads to an increase of total mitochondrial DNA while higher concentrations induce the mtDNA 4997 deletion in a human neuronal cell line
Authors: von Wurmb-Schwark N, Cavelier L, Cortopassi GA.
Journal: Mutat Res (2006): 57

Acetylcholinesterase activity in rats experimentally demyelinated with ethidium bromide and treated with interferon beta
Authors: Mazzanti CM, Spanevello RM, Pereira LB, Goncalves JF, Kaizer R, Correa M, Ahmed M, Mazzanti A, Festugatto R, Graca DL, Morsch VM, Schetinger MR.
Journal: Neurochem Res (2006): 1027

Analysis of cycloheximide-induced apoptosis in human leukocytes: Fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide
Authors: Baskic D, Popovic S, Ristic P, Arsenijevic NN.
Journal: Cell Biol Int (2006): 924

Apyrase and 5'-nucleotidase activities in synaptosomes from the cerebral cortex of rats experimentally demyelinated with ethidium bromide and treated with interferon-beta
Authors: Spanevello RM, Mazzanti CM, Kaizer R, Zanin R, Cargnelutti D, Hannel L, Correa M, Mazzanti A, Festugatto R, Graca D, Schetinger MR, Morsch VM.
Journal: Neurochem Res (2006): 455

Atomic force microscopic examination of chromosomes treated with trypsin or ethidium bromide
Authors: Wu Y, Cai J, Cheng L, Yun K, Wang C, Chen Y.
Journal: Chem Pharm Bull (Tokyo) (2006): 501

Delayed Schwann cell and oligodendrocyte remyelination after ethidium bromide injection in the brainstem of Wistar rats submitted to streptozotocin diabetogenic treatment
Authors: Bondan EF, Lallo MA, Trigueiro AH, Ribeiro CP, Sinhorini IL, Graca DL.
Journal: Braz J Med Biol Res (2006): 637

Determination of the DNA-binding characteristics of ethidium bromide, proflavine, and cisplatin by flow injection analysis: usefulness in studies on antitumor drugs
Authors: Alonso A, Almendral MJ, Curto Y, Criado JJ, Rodriguez E, Manzano JL.
Journal: Anal Biochem (2006): 157

Differential pulse voltammetric studies of ethidium bromide binding to DNA
Authors: Minasyan SH, Tavadyan LA, Antonyan AP, Davtyan HG, Parsadanyan MA, Vardevanyan PO.
Journal: Bioelectrochemistry (2006): 48

Ethidium bromide inhibits rat brain acetylcholinesterase activity in vitro
Authors: Mazzanti CM, Spanevello RM, Obregon A, Pereira LB, Streher CA, Ahmed M, Mazzanti A, Graca DL, Morsch VM, Schetinger MR.
Journal: Chem Biol Interact (2006): 121

Ethidium bromide is good not only for staining of nucleic acids but also for staining of proteins after polyacrylamide gel soaking in trichloroacetic acid solution
Authors: Dvortsov IA, Lunina NA, Chekanovskaya LA, Shedova EN, Gening LV, Velikodvorskaya GA.
Journal: Anal Biochem (2006): 293



说明书
DAPI乳酸 CAS 28718-91-4.pdf