DAPI(4',6-二脒基-2-苯基吲哚二盐酸盐)CAS 28718-90-3

货号17513存储条件f/l
规格25 mg价格1200
Ex (nm)359Em (nm)457
分子量350.25溶剂Water
产品详细介绍


简要概述

产品基本信息

货号:17513

产品名称:DAPI[4,6-二脒基-2-苯基吲哚,二盐酸盐]* 10 mM水溶液*

规格:25mg

储存条件:保存在冰箱-20℃干燥

保质期:6个月

 

产品物理化学光谱特性

分子量:350.25

溶剂:水

Ex(nm):358

Em(nm):461

 

产品介绍

        DAPI 4,6-二脒基-2-苯基吲哚,二盐酸盐是美国AAT Bioquest生产的用于染色DNA的试剂,DAPI是一种与DNA结合强烈的荧光染色剂。它广泛用于荧光显微镜。由于DAPI通过完整的细胞膜,它可用于除固定细胞外的活细胞染色。对于荧光显微镜,DAPI被紫外线激发。当与双链DNA结合时,其最大吸收在358nm,其发射最大值在461nm。DAPI的一个缺点是其排放相当广泛。DAPI也与RNA结合,但是它不像它与DNA结合时那样强烈地发荧光。 当与RNA结合时,其发射偏移至约500nm .DAPI的蓝色发射便于多重测定,因为DAPI与荧光素和绿色荧光蛋白(GFP)等绿色荧光分子或红色荧光染料之间的荧光重叠非常少。除了标记细胞核外,DAPI还用于检测细胞培养物中的支原体或病毒DNA。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的DNA染色试剂/试剂盒。 

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参考文献

BLT1 mediates commensal bacteria-dependent innate immune signals to enhance antigen-specific intestinal IgA responses.
Authors: T Nagatake, SI Hirata, T Koga, E Kuroda, S Kobari, H Suzuki, K Hosomi, N Matsumoto, Y Yanrismet, M Shimojou
Journal: Mucosal immunology (2019)

Low-Luminance Blue Light-Enhanced Phototoxicity in A2E-Laden RPE Cell Cultures and Rats
Authors: Cheng-Hui Lin, Man-Ru Wu, Wei-Jan Huang, Diana Shu-Lian Chow, George Hsiao, Yu-Wen Cheng
Journal: International journal of molecular sciences (2019): 1799

β-tricalcium phosphate composite ceramics with high compressive strength, enhanced osteogenesis and inhibited osteoclastic activities
Authors: Ye Tian, Teliang Lu, Fupo He, Yubin Xu, Haishan Shi, Xuetao Shi, Fei Zuo, Shanghua Wu, Jiandong Ye
Journal: Colloids and Surfaces B: Biointerfaces (2018)

δ-Tocopherol promotes thermogenic gene expression via PGC-1α upregulation in 3T3-L1 cells
Authors: Rieko Tanaka-Yachi, Masato Shirasaki, Rena Otsu, Chie Takahashi-Muto, Hideki Inoue, Yoshinori Aoki, Taisuke Koike, Chikako Kiyose
Journal: Biochemical and biophysical research communications (2018)

A Novel Heterozygous Missense Mutation in GNAT1 Leads to Autosomal Dominant Riggs Type of Congenital Stationary Night Blindness
Authors: Christina Zeitz, Cécile Méjécase, Mathilde Stévenard, Christelle Michiels, Isabelle Audo, Michael F Marmor
Journal: BioMed Research International (2018)

Acrylamide-induced oxidative stress and inflammatory response are alleviated by N-acetylcysteine in PC12 cells: Involvement of the crosstalk between Nrf2 and NF-$\kappa$B pathways regulated by MAPKs
Authors: Xiaoqi Pan, Xu Wu, Dandan Yan, Cheng Peng, Chaolong Rao, Hong Yan
Journal: Toxicology Letters (2018)

Augmenter of liver regeneration promotes mitochondrial biogenesis in renal ischemia--reperfusion injury
Authors: Li-li Huang, Rui-ting Long, Gui-ping Jiang, Xiao Jiang, Hang Sun, Hui Guo, Xiao-hui Liao
Journal: Apoptosis (2018): 1--12

Glucocorticoid-activation system mediated glucocorticoid-insulin-like growth factor 1 (GC-IGF1) axis programming alteration of adrenal dysfunction induced by prenatal caffeine exposure
Authors: Zheng He, Jinzhi Zhang, Hegui Huang, Chao Yuan, Chunyan Zhu, Jacques Magdalou, Hui Wang
Journal: Toxicology Letters (2018)

In vivo tailor-made protein corona of prodrug-based nanoassembly fabricated by redox dual-sensitive paclitaxel prodrug for the superselective treatment of breast cancer
Authors: Dong Zhang, Jincheng Yang, Jibin Guan, Bin Yang, Shenwu Zhang, Mengchi Sun, Ruitao Yang, Tao Zhang, Ruoshi Zhang, Qiming Kan
Journal: Biomaterials Science (2018)

Inactivation of the tight junction gene CLDN11 by aberrant hypermethylation modulates tubulins polymerization and promotes cell migration in nasopharyngeal carcinoma
Authors: Hsin-Pai Li, Chen-Ching Peng, Chih-Ching Wu, Chien-Hsun Chen, Meng-Jhe Shih, Mei-Yuan Huang, Yi-Ru Lai, Yung-Li Chen, Ting-Wen Chen, Petrus Tang
Journal: Journal of Experimental & Clinical Cancer Research (2018): 102

 

相关产品

产品名称 货号
DAPI(4',6-二脒基-2-苯基吲哚二盐酸盐)*10mM水溶液* Cat#17507


说明书
DAPI(4',6-二脒基-2-苯基吲哚二盐酸盐)CAS 28718-90-3.pdf