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细胞核染料Hoechst 33342 20mM水溶液 CAS 875756-97-1

英文名称:Hoechst 33342 *20 mM solution in water*
产品参数
Ex (nm)352Em (nm)454
分子量561.93溶剂Water
存储条件在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:Hoechst 33342细胞核染料

CAS:875756-97-1

储存条件:-20℃干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:561.93

外观:亮黄色粉末

溶剂:水或DMSO

激发波长(nm):350

发射波长(nm):461

 

产品介绍

Hoechst 染料是一系列用于荧光显微镜标记 DNA 的荧光染料。因为这些荧光染料可以标记 DNA,所以它们也常用于可视化细胞核和线粒体。其中两种密切相关的双苯并咪唑衍生物常被使用:Hoechst 33258 和 Hoechst 33342。这两种染料都在大约 350 纳米的紫外线下激发,并在 461 纳米的发射峰处发出蓝色/青色的荧光。Hoechst 染料可以用于活细胞或固定细胞,通常用作另一种核酸染料 DAPI 的替代品。它们之间的主要区别是 Hoechst 33342 添加的乙基使其更亲脂,因此更能穿透完整的细胞膜。在某些应用中,Hoechst 33258 的渗透性较低。这些染料还可以通过绘制标准的发射量与内容曲线来检测样本 DNA 的含量。

点击查看光谱

 

溶解方案(溶剂以说明书为准)

  0.1 mg 0.5 mg 1 mg 5 mg 10 mg
1 mM 177.958 µL 889.791 µL 1.78 mL 8.898 mL 17.796 mL
5 mM 35.592 µL 177.958 µL 355.916 µL 1.78 mL 3.559 mL
10 mM 17.796 µL 88.979 µL 177.958 µL 889.791 µL 1.78 mL
实验方案

染色细胞分析方案       

        以下程序适用于大多数细胞类型。 生长培养基,细胞密度,细胞类型和其他因素可能影响染色。 玻璃器皿上的残留洗涤剂也可能影响许多生物染色,导致在没有细胞存在的溶液中出现明亮染色的物质。

        通过离心沉淀细胞并将细胞重悬于缓冲盐溶液或培养基中,经过测试染料的佳结合pH为7.4。培养物中的贴壁细胞可以在盖玻片上或细胞培养孔中原位染色。Hoechst的染色浓度为0.5-5uM,并将其孵育15至60分钟。在初的实验中,好在整个建议范围内尝试几种染料浓度,以确定产生佳染色的浓度。

 

产品应用文献

Biofilm formation on the surface of monazite and xenotime during bioleaching
Authors: van Alin, Arya and Corbett, Melissa K and Fathollahzadeh, Homayoun and Tjiam, M Christian and Rickard, William DA and Sun, Xiao and Putnis, Andrew and Eksteen, Jacques and Kaksonen, Anna H and Watkin, Elizabeth
Journal: Microbial Biotechnology (2023)
CHST11-modified chondroitin 4-sulfate as a potential therapeutic target for glioblastoma
Authors: Lin, You-Cheng and Chu, Yin-Hung and Liao, Wen-Chieh and Chen, Chia-Hua and Hsiao, Wen-Chuan and Ho, Ying-Jui and Yang, Meng-Yin and Liu, Chiung-Hui
Journal: American Journal of Cancer Research (2023): 2998
TLR7 neo-functionalizes to sense dsRNA and trigger antiviral and antibacterial immunity in non-tetrapod vertebrates
Authors: Jiang, Rui and Zhu, Wentao and Liao, Zhiwei and Yang, Chunrong and Su, Jianguo
Journal: iScience (2023): 108315
Repair Kinetics of DSB-Foci Induced by Proton and $\alpha$-Particle Microbeams of Different Energies
Authors: Belchior, Ana and Canhoto, Jo{\~a}o F and Giesen, Ulrich and Langner, Frank and Rabus, Hans and Schulte, Reinhard
Journal: Life (2022): 2040
Repair kinetics of DSB-foci induced by proton and helium ion microbeams of different energies
Authors: Belchior, Ana and Canhoto, Jo{\~a}o F and Giesen, Ulrich and Langner, Frank and Rabus, Hans and Schulte, Reinhard
Journal: arXiv preprint arXiv:2206.08981 (2022)
NAMPT Inhibitor and P73 Activator Represses P53 R175H Mutated HNSCC Cell Proliferation in a Synergistic Manner
Authors: Cai, Bi-He and Bai, Zhi-Yu and Lien, Ching-Feng and Yu, Si-Jie and Lu, Rui-Yu and Wu, Ming-Han and Wu, Wei-Chen and Chen, Chia-Chi and Hsu, Yi-Chiang
Journal: Biomolecules (2022): 438
pH-responsive biopolymer-based supramolecular architectures as biodegradable carriers for 1 DOX delivery 2
Authors: Nia, Marzieh Heidari and Ashkar, Said and Munguia-Lopez, Jose G and Kinsella, Joseph M and van de Ven, Theo GM
Journal: (2021)
Biological and molecular characterization of a sheep pathogen isolate of Mannheimia haemolytica and leukotoxin production kinetics
Authors: Bkiri, Dounia and Semmate, Noha and Boumart, Zineb and Safini, Najete and Fakri, Fatima Zohra and Bamouh, Zahra and Tadlaoui, Khalid Omari and Fellahi, Siham and Tligui, Noursaid and Fihri, Ouafaa Fassi and others,
Journal: (2021)
Stimulation of $\alpha$7-nAChRs coordinates autophagy and apoptosis signaling in experimental knee osteoarthritis
Authors: Liu, Yuan and Xu, Shi and Zhang, Haijun and Qian, Kaoliang and Huang, Jiachen and Gu, Xianger and Li, Yan and Fan, Yi and Hu, Jun
Journal: Cell Death \& Disease (2021): 1--12
Microencapsulated Multi-functionalized Graphene Oxide Equipped With Chloroquine for Efficient and Sustained Sirna Delivery
Authors: Imani, Rana and Prakash, Satya and Vali, Hojatollah and Presley, John F and Faghihi, Shahab
Journal: (2021)
 

参考文献

Usefulness of a triple fluorochrome combination Merocyanine 540/Yo-Pro 1/Hoechst 33342 in assessing membrane stability of viable frozen-thawed spermatozoa from Estonian Holstein AI bulls
Authors: Hallap T, Nagy S, Jaakma U, Johannisson A, Rodriguez-Martinez H.
Journal: Theriogenology (2006): 1122
 
Fatty acid synthase and its mRNA concentrations are decreased at different times following Hoechst 33342-induced apoptosis in BC3H-1 myocytes
Authors: Zhang X, Kiechle FL.
Journal: Ann Clin Lab Sci (2006): 185
 
The DNA minor groove binding agents Hoechst 33258 and 33342 enhance recombinant adeno-associated virus (rAAV) transgene expression
Authors: Li L, Yang L, Kotin RM.
Journal: J Gene Med (2005): 420
 
Resistance mechanism development to the topoisomerase-I inhibitor Hoechst 33342 by Leishmania donovani
Authors: Marquis JF, Hardy I, Olivier M.
Journal: Parasitology (2005): 197
 
Acid-base and electronic structure-dependent properties of Hoechst 33342
Authors: Aleman C, Namba AM, Casanovas J.
Journal: J Biomol Struct Dyn (2005): 29
 
Single UV excitation of Hoechst 33342 and propidium iodide for viability assessment of rhesus monkey spermatozoa using flow cytometry
Authors: Cai K, Yang J, Guan M, Ji W, Li Y, Rens W.
Journal: Arch Androl (2005): 371
 
Evidence for a qualitative hierarchy within the Hoechst-33342 'side population' (SP) of murine bone marrow cells
Authors: Robinson SN, Seina SM, Gohr JC, Kuszynski CA, Sharp JG.
Journal: Bone Marrow Transplant (2005): 807
 
Flow cytometric characterization of viable meiotic and postmeiotic cells by Hoechst 33342 in mouse spermatogenesis
Authors: Bastos H, Lassalle B, Chicheportiche A, Riou L, Testart J, Allem and I, Fouchet P.
Journal: Cytometry A (2005): 40
 
An in vitro study of Hoechst 33342 redistribution and its effects on cell viability
Authors: Mohorko N, Kregar-Velikonja N, Repovs G, Gorensek M, Bresjanac M.
Journal: Hum Exp Toxicol (2005): 573
 
Role of topoisomerases in cytotoxicity induced by DNA ligand Hoechst-33342 and UV-C in a glioma cell line
Authors: Singh S, Dwarakanath BS, Lazar Mathew T.
Journal: Indian J Exp Biol (2005): 313