细胞核染料Hoechst 33342 20mM水溶液 CAS 875756-97-1
英文名称：Hoechst 33342 *20 mM solution in water*
产品参数
| Ex (nm) | 352 | Em (nm) | 454 |
| 分子量 | 561.93 | 溶剂 | Water |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品概述
产品货期
咨询
产品优势
1.常作为DAPI的替代染色方案
2.DNA特异性结合，实现精准核酸标记
适用范围
用于标记活细胞与固定细胞
产品介绍
Hoechst荧光染料是一类广泛应用于DNA标记的荧光染色剂家族，在荧光显微镜技术中具有重要作用。作为DNA特异性染料，它们不仅可用于细胞核显像，也常用于线粒体观察。目前最常用的两种双苯并咪唑类染料Hoechst 33258和Hoechst 33342具有相似的光谱特性：均在350nm紫外光激发下产生461nm的蓝/青色荧光发射峰。
这类染料既适用于活细胞也适用于固定细胞染色，常作为核酸染料DAPI的替代选择。它们之间的主要区别是 Hoechst 33342 添加的乙基使其更亲脂，因此更能穿透完整的细胞膜。在某些应用中，Hoechst 33258 的渗透性较低。此外，通过建立标准荧光强度-DNA含量曲线，这些染料还可用于样本DNA含量的定量分析。
溶解方案(溶剂以说明书为准)
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 177.958 µL | 889.791 µL | 1.78 mL | 8.898 mL | 17.796 mL |
| 5 mM | 35.592 µL | 177.958 µL | 355.916 µL | 1.78 mL | 3.559 mL |
| 10 mM | 17.796 µL | 88.979 µL | 177.958 µL | 889.791 µL | 1.78 mL |
实验方案
染色细胞分析方案
以下染色方案适用于大多数细胞类型，但需注意培养基成分、细胞密度、共培养细胞等因素均可能影响染色效果。实验器皿残留的洗涤剂可能导致假阳性染色（即便在没有细胞的溶液中也可能出现荧光物质）。
建议通过离心收集细胞后，用pH 7.4的缓冲盐溶液或培养基重悬（该pH条件最有利于染料结合）。贴壁细胞可直接在盖玻片或培养孔中原位染色。通常使用0.5-5 μM浓度范围的Hoechst染料，孵育15-60分钟。初次实验时，建议在推荐浓度范围内设置梯度实验以确定合适的染色条件。
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