核酸染料 LDS 751*CAS 181885-68-7*(停产)

货号17561存储条件 在零下15度以下保存, 避免光照
规格25 mg价格4740
Ex (nm)561Em (nm)712
分子量471.98溶剂DMSO
产品详细介绍


简要概述

产品基本信息

货号:17561

产品名称:核酸染料LDS 751

规格:25mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:471.98

外观:深绿色粉末

溶剂:水或DMSO

激发波长(nm):543

发射波长(nm):712

 

产品介绍

LDS 751是一种渗透性细胞核酸染料,它在dsDNA上在〜543 nm处具有峰值激发,但可以用488 nm激光很好地激发。它是DRAQ 5 的替代产品,由于其最大的最大波长发射波长(〜712 nm),可用于多色分析。与LDS 751的dsDNA结合后,荧光增强程度约为20倍。LDS-751不进入细胞核,并与线粒体膜结合。因此,当使用LDS 751分析活细胞和使用LDS-751探针检测细胞时需要清楚,它不可用于细胞核检测。噻唑橙(RNA染料)和LDS-751(DNA染料)组合物已被用于将网织红细胞与其他细胞群分离。 LDS 751也已用于从有核细胞中分离红细胞。

点击查看光谱

 

参考文献

Exciton-controlled fluorescence: application to hybridization-sensitive fluorescent DNA probe
Authors: Okamoto A, Ikeda S, Kubota T, Yuki M, Yanagisawa H.
Journal: Nucleic Acids Symp Ser (Oxf) (2009): 49

Hybridization-sensitive fluorescent probe for long-term monitoring of intracellular RNA
Authors: Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A.
Journal: Bioconjug Chem (2009): 1256

Interaction of LDS-751 with the drug-binding site of P-glycoprotein: a Trp fluorescence steady-state and lifetime study
Authors: Lugo MR, Sharom FJ.
Journal: Arch Biochem Biophys (2009): 17

Flow cytometric assessment of homeostatic aging of reticulocytes in rats
Authors: Wiczling P, Krzyzanski W.
Journal: Exp Hematol (2008): 119

Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry
Authors: Maes ML, Davidson LB, McDonagh PF, Ritter LS.
Journal: J Immunol Methods (2007): 79

Determination of the formation of dark state via depleted spontaneous emission in a complex solvated molecule
Authors: Guo X, Wang S, Xia A, Su H.
Journal: J Phys Chem A (2007): 5800

Cell cycle synchronization of Cupriavidus necator by continuous phasing measured via flow cytometry
Authors: Fritsch M, Starruss J, Loesche A, Mueller S, Bley T.
Journal: Biotechnol Bioeng (2005): 635

Exciton mobility and trapping in a MALDI matrix
Authors: Setz PD, Knochenmuss R.
Journal: J Phys Chem A (2005): 4030

Interaction of LDS-751 and rhodamine 123 with P-glycoprotein: evidence for simultaneous binding of both drugs
Authors: Lugo MR, Sharom FJ.
Journal: Biochemistry (2005): 14020

Quantitative evaluation of isothiocyanates as substrates and inhibitors of P-glycoprotein
Authors: Barecki-Roach M, Wang EJ, Johnson WW.
Journal: J Pharm Pharmacol (2003): 1251



产品说明书

染色细胞分析方案

注意:以下协议适用于大多数细胞类型。 生长培养基,细胞密度,其他细胞类型和因子的存在可能影响染色。 玻璃器皿上的残留洗涤剂也可能影响许多生物的染色,并导致在有或没有细胞存在的溶液中出现明亮染色的物质。

1.制备5-10 mM DMSO储备溶液。

2.向细胞(悬浮细胞或贴壁细胞)中加入1至10μM,并将细胞染色15至60分钟。 在最初的实验中,最好尝试几种染料浓度来确定产生所需结果的最佳浓度。 高染料浓度倾向于引起其他细胞结构的非特异性染色。

3.用荧光显微镜,荧光酶标仪或流式细胞仪直接分析细胞染色。

 

参考文献

Exciton-controlled fluorescence: application to hybridization-sensitive fluorescent DNA probe
Authors: Okamoto A, Ikeda S, Kubota T, Yuki M, Yanagisawa H.
Journal: Nucleic Acids Symp Ser (Oxf) (2009): 49

Hybridization-sensitive fluorescent probe for long-term monitoring of intracellular RNA
Authors: Kubota T, Ikeda S, Yanagisawa H, Yuki M, Okamoto A.
Journal: Bioconjug Chem (2009): 1256

Interaction of LDS-751 with the drug-binding site of P-glycoprotein: a Trp fluorescence steady-state and lifetime study
Authors: Lugo MR, Sharom FJ.
Journal: Arch Biochem Biophys (2009): 17

Flow cytometric assessment of homeostatic aging of reticulocytes in rats
Authors: Wiczling P, Krzyzanski W.
Journal: Exp Hematol (2008): 119

Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry
Authors: Maes ML, Davidson LB, McDonagh PF, Ritter LS.
Journal: J Immunol Methods (2007): 79

Determination of the formation of dark state via depleted spontaneous emission in a complex solvated molecule
Authors: Guo X, Wang S, Xia A, Su H.
Journal: J Phys Chem A (2007): 5800

Cell cycle synchronization of Cupriavidus necator by continuous phasing measured via flow cytometry
Authors: Fritsch M, Starruss J, Loesche A, Mueller S, Bley T.
Journal: Biotechnol Bioeng (2005): 635

Exciton mobility and trapping in a MALDI matrix
Authors: Setz PD, Knochenmuss R.
Journal: J Phys Chem A (2005): 4030

Interaction of LDS-751 and rhodamine 123 with P-glycoprotein: evidence for simultaneous binding of both drugs
Authors: Lugo MR, Sharom FJ.
Journal: Biochemistry (2005): 14020

Quantitative evaluation of isothiocyanates as substrates and inhibitors of P-glycoprotein
Authors: Barecki-Roach M, Wang EJ, Johnson WW.
Journal: J Pharm Pharmacol (2003): 1251



说明书
核酸染料 LDS 751*CAS 181885-68-7*(停产).pdf