Gelite Safe核酸凝胶染料* 10,000X水溶液*

货号17700存储条件 在零下15度以下保存, 避免光照
规格100 ul价格900
Ex (nm)513Em (nm)552
分子量N/A溶剂Water
产品详细介绍


简要概述

Gelite Safe 核酸凝胶染料是一种非常敏感且稳定的荧光染料,可用于电泳凝胶中的DNA检测。 与高毒性的溴化乙锭(EtBr)不同,Gelite Safe经过专门设计,具有较低的危害性,无细胞毒性和诱变性,与EtBr和SYBR®Safe相比,具有更高的灵敏度和更低的背景荧光。 Gelite Safe的独特光谱特性扩展了其仪器兼容性,包括紫外和蓝光透射仪,凝胶记录系统和激光扫描仪。无需繁杂的操作,可在在绿色或红色通道中进行检测。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的Gelite Safe核酸凝胶染料* 10,000X水溶液*。 

点击查看光谱

 

适用仪器


凝胶成像仪  
激发(nm): 紫外透射仪/蓝色激发光
发射(nm): SYBR® 滤波片, GelStar® 滤波片, GelGreen® 滤波片, or GelRed® 滤波片


产品说明书

样品实验方案

工作溶液配制

Gelite Safe 工作溶液

在7.5-8.5的pH范围内使用(例如,TAE、TBE或TE pH 8.2)缓冲液稀释10,000X储备试剂来制备1X Gelite Safe工作溶液。
注意:在水中配制的染色溶液比在缓冲液中配制的溶液不稳定,必须在24小时内使用。

 

后染色方案
1.根据您的标准方案运行凝胶。
2.将凝胶放在合适的聚丙烯容器中。 轻轻加入足量的1X染色溶液以浸没凝胶。
注意:请勿使用玻璃容器,因为它会吸收染色溶液中的许多染料。
3.在室温下避光轻轻搅拌凝胶约30至60分钟。
4.使用300 nm / 254 nm紫外透射仪或使用长距离绿色滤光片(例如SYBR®滤光片,GelStar®滤光片,GelGreen®滤光片或GelRed®滤光片)的凝胶扫描仪对凝胶成像。

 

预染色方案
1.使用标准方案准备琼脂糖凝胶溶液。
2.在倒入凝胶之前,将10,000X Gelite Safe储备试剂以1:10,000的比例稀释到凝胶溶液中并彻底混合。
3.根据您的标准方案运行凝胶。
4.使用300 nm / 254 nm紫外透射仪或使用长距离绿色滤光片(例如SYBR®滤光片,GelStar®滤光片,GelGreen®滤光片或GelRed®滤光片)的凝胶扫描仪对凝胶成像。

 

图示

图1.使用Gelite Safe,EtBr和SYBR®Safe在TBE缓冲液中的1%琼脂糖凝胶中检测DNA的比较。 从左到右分别以100 ng,50 ng和25 ng的量加载1 kb DNA的两倍连续稀释液。 根据制造商推荐的浓度,将凝胶用Gelite Safe,EtBr和SYBR®Safe染色60分钟,并使用ChemiDoc 成像系统(Bio-Rad®)进行成像。 使用装有GelGreen和GelRed滤光片的300 nm荧光透射仪对凝胶进行照明。

 

参考文献

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说明书
Gelite Safe核酸凝胶染料* 10,000X水溶液*.pdf