ProLite 橙色蛋白凝胶染料* 5000X *

货号18000存储条件 在零下15度以下保存, 避免光照
规格100 ul价格1140
Ex (nm)484Em (nm)586
分子量486.72溶剂Water
产品详细介绍


简要概述

Prolite Orange是一种蛋白凝胶染料,可用于替代SYPRO Orange蛋白凝胶染料(SYPRO是ThermoFisher的商标)。 Prolite Orange是一种灵敏的即用型荧光染料,可在一维凝胶中检测总蛋白。 Prolite Orange的灵敏度比传统的银染技术更好。 可以使用标准的UV或蓝光透射仪或包含适当滤镜或激光的成像设备查看染色的蛋白质。 荧光染色快速且高度灵敏,可检测蛋白质电泳凝胶和膜中的总蛋白质。 

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产品说明书

样品分析方案

储存

存放在-20°C避光的地方。 按建议存放时,产品自收到之日起至少稳定12个月。 可用醋酸或缓冲液稀释的ProLite Orange可在4°C的玻璃或塑料瓶中保存三个月,避光。 打开之前,应先将每个小瓶加热至室温,然后在微量离心机中短暂离心,以将DMSO溶液沉积在小瓶底部。 如果存在染料颗粒,请短暂超声处理试管或剧烈涡旋试管。

 

工作溶液配制

ProLite Orange工作溶液(5000X)
用7.5%(v / v)乙酸稀释5000X ProLite Orange储备溶液,制成1X ProLite Orange储备溶液,并剧烈混合。
注意:工作溶液最多可以重复使用四次。 但是,我们观察到第二次重用后响应开始明显减少。 强烈建议使用全新的工作溶液以获得最佳效果。

 

操作步骤

建议使用以下方案,并且可以将其用作准则。但是,可能需要进行一些比较才能确定哪一种可以更好地满足您的需求。

 

电泳后染色蛋白

1.运行凝胶。
2.将工作溶液倒入一个小的塑料皿中。
注意:对于一到两个标准尺寸的小凝胶,请使用约50 mL的工作溶液。对于较大的凝胶,请使用500至700 mL的工作溶液。
注意:确保添加足够的工作量以完全浸没凝胶。
3.将凝胶放入工作溶液中。
注意:用铝箔盖住容器,以防止染料受光。
4.在室温下轻轻搅拌凝胶10至60分钟。
5.用7.5%的乙酸短暂冲洗。
6.可以在标准的300 nm紫外线透射仪或蓝光透射仪上观察凝胶。
7.脱色:大部分凝胶可通过在0.1%Tween®20中孵育过夜来脱色。或者,在7.5%乙酸的多种变化中孵育最终将去除所有污渍。

 

参考文献

Fluorescent thermal shift-based method for detection of NF-κB binding to double-stranded DNA.
Authors: Leitner, Peter D and Vietor, Ilja and Huber, Lukas A and Valovka, Taras
Journal: Scientific reports (2021): 2331

Ligand binding to a humanized anti-cocaine mAb measured by dye absorption spectroscopy.
Authors: Kirley, Terence L and Norman, Andrew B
Journal: Biochemical and biophysical research communications (2021): 93-98

Thermal Shift Assay for Exploring Interactions Between Fatty Acid-Binding Protein and Inhibitors.
Authors: Hao, Jiaqing
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 395-409

Thermal shift assay to probe melting of thrombin, fibrinogen, fibrin monomer, and fibrin: Gly-Pro-Arg-Pro induces a fibrin monomer-like state in fibrinogen.
Authors: Crossen, J and Diamond, S L
Journal: Biochimica et biophysica acta. General subjects (2021): 129805

A novel differential scanning fluorimetry analysis of a humanized anti-cocaine mAb and its ligand binding characteristics.
Authors: Kirley, Terence L and Norman, Andrew B and Wetzel, Hanna N
Journal: Journal of immunological methods (2020): 112676

Intrinsic Differential Scanning Fluorimetry for Fast and Easy Identification of Adeno-Associated Virus Serotypes.
Authors: Rieser, Ruth and Penaud-Budloo, Magalie and Bouzelha, Mohammed and Rossi, Axel and Menzen, Tim and Biel, Martin and Büning, Hildegard and Ayuso, Eduard and Winter, Gerhard and Michalakis, Stylianos
Journal: Journal of pharmaceutical sciences (2020): 854-862

SYPRO Orange - a new gold standard amyloid probe.
Authors: Mora, Aruna K and Nath, Sukhendu
Journal: Journal of materials chemistry. B (2020): 7894-7898

nanoDSF: In vitro Label-Free Method to Monitor Picornavirus Uncoating and Test Compounds Affecting Particle Stability.
Authors: Real-Hohn, Antonio and Groznica, Martin and Löffler, Nadine and Blaas, Dieter and Kowalski, Heinrich
Journal: Frontiers in microbiology (2020): 1442

Destructive twisting of neutral metalloproteases: the catalysis mechanism of the Dispase autolysis-inducing protein from Streptomyces mobaraensis DSM 40487.
Authors: Fiebig, David and Storka, Juliana and Roeder, Markus and Meyners, Christian and Schmelz, Stefan and Blankenfeldt, Wulf and Scrima, Andrea and Kolmar, Harald and Fuchsbauer, Hans-Lothar
Journal: The FEBS journal (2018): 4246-4264

Evaluation of fluorescent dyes to measure protein aggregation within mammalian cell culture supernatants.
Authors: Oshinbolu, Sheun and Shah, Rachana and Finka, Gary and Molloy, Mike and Uden, Mark and Bracewell, Daniel G
Journal: Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986) (2018): 909-917



说明书
ProLite 橙色蛋白凝胶染料* 5000X *.pdf