FluoroQuest 防褪色固定介质(含DAPI)

货号20005存储条件 在2-8度冷藏保存, 避免光照
规格20 mL价格2472
Ex (nm)359Em (nm)457
分子量溶剂Water
产品详细介绍


简要概述

具有DAPI的FluoroQuest 是一种水性固定介质,用于保留组织和细胞涂片的荧光。这种封固剂含有抗褪色剂以降低染料的光漂白速率,并以DAPI强化,DAPI是DNA的复染剂。用于原位杂交技术或需要荧光法进行DNA染色的其他方法。DAPI在360 nm处激发并在460 nm处发射,产生蓝色荧光。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的FluoroQuest 防褪色固定介质。 

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产品说明书

样品实验方案

以下是我们推荐的方案,仅提供指导。具体实验应根据您的特定需求进行修改。

  1. 预热溶液:从冰箱中取出安装溶液。将瓶子加热至室温,避光。
  2. 添加溶液:清除样品中多余的液体。向样品中加一滴固定溶液。如果样品在载玻片或组织培养皿上,请小心地将盖玻片放在液滴上,以免产生气泡。如果样品在盖玻片上,请将盖玻片倒在干净的玻璃载玻片上。去除多余的防褪色成分。
  3. 准备用于成像的样品:固定溶液应孵育2小时至过夜。要长期存放,请用指甲油或塑料密封剂将盖玻片密封在玻片上。已安装的载玻片应在黑暗中于4°C存放,荧光成像将保持稳定数周,样品可以在安装后立即成像。 

 

图示

图1. 甲醛固定,石蜡包埋的人肺腺癌阳性组织的荧光IHC 。用兔抗EpCam抗体对人肺腺癌阳性组织切片进行染色,然后分别与iFluor 488 Styramide (Cat#45020)染色的聚HRP标记的羊抗兔IgG二抗一起孵育。将组织固定在带有DAPI(Cat#20005)的FluoroQuest 抗褪色固定介质中。

 

参考文献

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A Power-Efficient Clustering Protocol for Coal Mine Face Monitoring with Wireless Sensor Networks Under Channel Fading Conditions.
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FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6.
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Production of colourful pigments consisting of amorphous arrays of silica particles.
Authors: Yoshioka, Shinya and Takeoka, Yukikazu
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A dendritic single-molecule fluorescent probe that is monovalent, photostable and minimally blinking.
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Journal: Nature chemistry (2013): 692-7

A comparison of fluorescent stains for the assessment of viability and metabolic activity of lactic acid bacteria.
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Anti-fading media for live cell GFP imaging.
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说明书
FluoroQuest 防褪色固定介质(含DAPI).pdf