钙离子荧光探针Cal Red R525/650 AM

货号20590存储条件 在零下15度以下保存, 避免光照
规格1 mg价格6432
Ex (nm)492Em (nm)650
分子量~1100溶剂DMSO
产品详细介绍


简要概述

产品基本信息

货号:20590

产品名称:钙离子荧光探针Cal Red R525/650 AM

规格:1mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:~1100

溶剂:DMSO

激发波长(nm):492

发射波长(nm):650

 

适用仪器


荧光显微镜  
激发: FITC
发射: FITC
推荐孔板: 黑色透明
荧光酶标仪  
激发: 490nm
发射: 525,660nm
cutoff: 515,630nm
推荐孔板: 黑色透明
读取模式: 底读模式

 

产品介绍

钙离子荧光探针Cal Red R525/650 AM是美国AAT Bioquest生产的钙离子荧光探针,细胞内钙通量测定是一种广泛使用的监测GPCR和钙通道活性的方法。为了量化细胞内钙浓度,比率荧光钙指示剂是优选的,因为该比例与钙浓度直接相关并且与细胞数量和染料加载浓度无关。然而,最流行的比率钙指示剂(例如Fura-2和Indo-1)具有某些限制,例如较低的灵敏度,UV激发,并且与HTS筛选过滤器组不兼容。 Cal Red™R525 / 650已被开发为新的488 nm可激发的比率荧光钙指示剂。 Cal Red™R525 / 650是弱荧光的,一旦进入细胞,亲脂性AM阻断基团被细胞内酯酶切割,导致带负电荷的荧光染料在细胞中保留良好,激发接近488 nm,两次发射在525 nm处, 650纳米。当用生物活性化合物刺激细胞时,该受体启动细胞内钙的释放,其被Cal Red TM R525 / 650螯合。当在488nm激发时,发射信号在525nm处增加并在650nm处减小。 Cal Red™R525 / 650的激发和发射波长与普通滤光片组兼容,对细胞的损伤最小,使其成为评估和筛选GPCR激动剂和拮抗剂以及钙通道靶标的有力工具。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的钙离子荧光探针Cal Red R525/650 AM。 

点击查看实验方案

钙离子篇:时间轴式讲解应用于钙离子检测的探针

 

参考文献

Ratiometric analysis of fura red by flow cytometry: a technique for monitoring intracellular calcium flux in primary cell subsets
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Journal: PLoS One (2015): e0119532

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux
Authors: Bailey S, Macardle PJ.
Journal: J Immunol Methods (2006): 220

Use of co-loaded Fluo-3 and Fura Red fluorescent indicators for studying the cytosolic Ca(2+)concentrations distribution in living plant tissue
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Journal: Cell Calcium (2000): 23

[Monitoring calcium in outer hair cells with confocal microscopy and fluorescence ratios of fluo-3 and fura-red]
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Journal: Shi Yan Sheng Wu Xue Bao (1998): 323

Calcium transient alternans in blood-perfused ischemic hearts: observations with fluorescent indicator fura red
Authors: Wu Y, Clusin WT.
Journal: Am J Physiol (1997): H2161

Problems associated with using Fura-2 to measure free intracellular calcium concentrations in human red blood cells
Authors: Blackwood AM, Sagnella GA, Markandu ND, MacGregor GA.
Journal: J Hum Hypertens (1997): 601

IgG-induced Ca2+ oscillations in differentiated U937 cells; a study using laser scanning confocal microscopy and co-loaded fluo-3 and fura-red fluorescent probes
Authors: Floto RA, Mahaut-Smith MP, Somasundaram B, Allen JM.
Journal: Cell Calcium (1995): 377

Improved sensitivity in flow cytometric intracellular ionized calcium measurement using fluo-3/Fura Red fluorescence ratios
Authors: Novak EJ, Rabinovitch PS.
Journal: Cytometry (1994): 135

Localization of calcium entry through calcium channels in olfactory receptor neurones using a laser scanning microscope and the calcium indicator dyes Fluo-3 and Fura-Red
Authors: Schild D, Jung A, Schultens HA.
Journal: Cell Calcium (1994): 341

The distribution of intracellular calcium chelator (fura-2) in a population of intact human red cells
Authors: Lew VL, Etzion Z, Bookchin RM, daCosta R, Vaananen H, Sassaroli M, Eisinger J.
Journal: Biochim Biophys Acta (1993): 152



说明书
钙离子荧光探针Cal Red R525/650 AM.pdf