新型钙离子荧光探针Calbryte 630, AM *细胞渗透性*

货号20721存储条件 在零下15度以下保存, 避免光照
规格10x50 ug价格5136
Ex (nm)607Em (nm)624
分子量1234.84溶剂DMSO
产品详细介绍


简要概述

产品基本信息

货号:20721

产品名称:新型钙离子荧光探针Calbryte 630, AM *细胞渗透性*

规格:10x50 ug

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:1234.84

溶剂:DMSO

激发波长(nm):608

发射波长(nm):626

 

适用仪器


流式细胞仪  
激发: 640 nm 激光
发射: 660/20 nm 滤波片
通道: APC 通道
荧光显微镜  
激发: Texas Red
发射: Texas Red
推荐孔板: 黑色透明
荧光酶标仪  
激发: 600nm
发射: 640nm
cutoff: 630nm
推荐孔板: 黑色透明
读取模式: 底读模式

 

产品介绍

新型钙离子荧光探针Calbryte 630, AM *细胞渗透性*是美国AAT Bioquest生产的钙离子荧光探针,钙测量对于许多生物学研究至关重要。显示结合钙后光谱响应的荧光探针使研究人员能够使用荧光显微镜,流式细胞仪,荧光光谱和荧光酶标仪来研究细胞内游离钙浓度的变化。x-Rhod-1通常用作红色荧光钙指示剂。但是,x-Rhod-1在酯酶水解后在活细胞中仅适度发荧光,并且细胞钙反应非常小。Calbryte 630的开发旨在改善x-Rhod-1的细胞负载和钙反应,同时保持x-Rhod-1的光谱波长,使其与TexasRed®滤光片兼容。在CHO和HEK细胞中,Cal-630 AM的细胞钙反应比x-Rhod-1敏感得多。Calbryte 630的光谱与FITC,AlexaFluor®488和GFP的光谱完全分离,使其成为与GFP细胞系或FITC / AlexaFluor®488标记的抗体进行细胞内分析多重检测的理想钙探针。Calbryte 630是用于测量细胞内钙的新一代红色荧光指示剂。Calbryte 630 AM的信号/背景比和细胞内保留特性大大提高,使其成为评估GPCR和钙通道靶标以及在活细胞中筛选其激动剂和拮抗剂的最强大的深红色荧光指示剂。像其他染料AM细胞上样一样,Calbryte 630 AM酯是非荧光的,一旦进入细胞内部,它就会被细胞内酯酶水解并被激活。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的新型钙离子荧光探针Calbryte 630, AM *细胞渗透性*。 

点击查看光谱

点击查看实验方案

钙离子篇:时间轴式讲解应用于钙离子检测的探针

 

参考文献

Ca2+ Diffusion in the Large Peptidergic Nerve Terminals of the Posterior Pituitary
Authors: Shane M McMahon, Meyer B Jackson
Journal: Biophysical Journal (2019): 238a

Functionally Identifying Members of the MscS Superfamily of Ion Channels in Paraburkholderia Membranes
Authors: Hannah M Dickinson, Brittni L Miller, Hannah R Malcolm
Journal: Biophysical Journal (2019): 240a--241a

LASS2 inhibits proliferation and induces apoptosis in HepG2 cells by affecting mitochondrial dynamics, the cell cycle and the nuclear factor-$\kappa$B pathways
Authors: Yan Yang, Xiaoli Yang, Lin Li, Gangyi Yang, Xuhong Ouyang, Jialin Xiang, Tao Zhang, Xun Min
Journal: Oncology reports (2019)

Null-Sarcolipin Equine Muscle Shows Enhanced SERCA Calcium Transport Which May Potentiate the Prevalence of Exertional Rhabdomyolysis
Authors: Joseph M Autry, Bengt Svensson, Christine B Karim, Sudeep Perumbakkam, Zhenhui Chen, Carrie J Finno, David D Thomas, Stephanie J Valberg
Journal: Biophysical Journal (2019): 238a--239a

Shear stress induced nuclear shrinkage through activation of Piezo1 channels in epithelial cells
Authors: Deekshitha Jetta, Philip A Gottlieb, Deepika Verma, Frederick Sachs, Susan Z Hua
Journal: J Cell Sci (2019): jcs--226076

The Arrhythmogenic E105A CAM Mutation Dysregulates Normal Cardiac Function in Zebrafish by Altering CAM-Ca2+ and CAM-RyR2 Interactions
Authors: Michail Nomikos, Sahar I Da’as, Angelos Thanassoulas, Rola Salem, Brian L Calver, Alaaeldin Saleh, Ali Al-Maraghi, Gheyath K Nasrallah, Bared Safieh-Garabedian, Egon Toft
Journal: Biophysical Journal (2019): 240a

Improvements in Simultaneous Sodium and Calcium Imaging
Authors: Kenichi Miyazaki, John E Lisman, William N Ross
Journal: Frontiers in cellular neuroscience (2018)

Calreticulin regulates TGF-β1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling
Authors: Yanjiao Wu, Xiaoli Xu, Lunkun Ma, Qian Yi, Weichao Sun, Liling Tang
Journal: The International Journal of Biochemistry & Cell Biology (2017)

Dexmedetomidine reduces hypoxia/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neurons
Authors: Jia Liu, Qing Du, He Zhu, Yu Li, Maodong Liu, Shoushui Yu, Shilei Wang
Journal: Int J Clin Exp Med (2017): 6861--6868

Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells
Authors: Jiang Lu, Xue-qin Yao, Xin Luo, Yu Wang, Sookja Kim Chung, He-xin Tang, Chi Wai Cheung, Xian-yu Wang, Chen Meng, Qing Li
Journal: Neural Regeneration Research (2017): 945

 

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说明书
新型钙离子荧光探针Calbryte 630, AM *细胞渗透性*.pdf