钙离子荧光探针Fura Red, AM

货号21046存储条件 在零下15度以下保存, 避免光照
规格1 mg价格4488
Ex (nm)435Em (nm)639
分子量1088.99溶剂DMSO
产品详细介绍


简要概述

产品基本信息

货号:21046

产品名称:钙离子荧光探针Fura Red, AM

规格:1mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:1088.99

溶剂:DMSO

激发波长(nm):458

发射波长(nm):597

 

适用仪器


荧光酶标仪  
激发: 435,470nm
发射: 630,650nm
cutoff: Ex/Em = 435/630, cutoff 610. Ex/Em = 470/650, cut off 630
推荐孔板: 黑色透明
读取模式: 底读模式

 

产品介绍

钙离子荧光探针Fura Red, AM是美国AAT Bioquest生产的钙离子荧光探针,Fura Red是一种可见光可激发的fura-2类似物,当与单激发,绿色荧光钙指示剂一起使用时,通过显微光度法,成像或流式细胞术为单细胞中的钙离子比率测量提供了独特的可能性。 Fura Red AM是用于非侵入性细胞内负载的Fura Red的细胞渗透型。 Fura Red AM可以同时装入Fluo-3 AM,Fluo-8 AM或Cal-520 AM的细胞中。 组合两种钙染料的优点是可以使用具有较长激发波长的染料。 与使用用UV或近紫外光(例如Fura-2)激发的比率染料相比,这通常对细胞造成的伤害更小,因为可见光波长的光具有较低的光毒性。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的钙离子荧光探针Fura Red, AM。 

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钙离子篇:时间轴式讲解应用于钙离子检测的探针

 

参考文献

Ratiometric analysis of fura red by flow cytometry: a technique for monitoring intracellular calcium flux in primary cell subsets
Authors: Wendt ER, Ferry H, Greaves DR, Keshav S.
Journal: PLoS One (2015): e0119532

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux
Authors: Bailey S, Macardle PJ.
Journal: J Immunol Methods (2006): 220

Use of co-loaded Fluo-3 and Fura Red fluorescent indicators for studying the cytosolic Ca(2+)concentrations distribution in living plant tissue
Authors: Walczysko P, Wagner E, Albrechtova JT.
Journal: Cell Calcium (2000): 23

[Monitoring calcium in outer hair cells with confocal microscopy and fluorescence ratios of fluo-3 and fura-red]
Authors: Su ZL, Li N, Sun YR, Yang J, Wang IM, Jiang SC.
Journal: Shi Yan Sheng Wu Xue Bao (1998): 323

Calcium transient alternans in blood-perfused ischemic hearts: observations with fluorescent indicator fura red
Authors: Wu Y, Clusin WT.
Journal: Am J Physiol (1997): H2161

Problems associated with using Fura-2 to measure free intracellular calcium concentrations in human red blood cells
Authors: Blackwood AM, Sagnella GA, Markandu ND, MacGregor GA.
Journal: J Hum Hypertens (1997): 601

IgG-induced Ca2+ oscillations in differentiated U937 cells; a study using laser scanning confocal microscopy and co-loaded fluo-3 and fura-red fluorescent probes
Authors: Floto RA, Mahaut-Smith MP, Somasundaram B, Allen JM.
Journal: Cell Calcium (1995): 377

Improved sensitivity in flow cytometric intracellular ionized calcium measurement using fluo-3/Fura Red fluorescence ratios
Authors: Novak EJ, Rabinovitch PS.
Journal: Cytometry (1994): 135

Localization of calcium entry through calcium channels in olfactory receptor neurones using a laser scanning microscope and the calcium indicator dyes Fluo-3 and Fura-Red
Authors: Schild D, Jung A, Schultens HA.
Journal: Cell Calcium (1994): 341

The distribution of intracellular calcium chelator (fura-2) in a population of intact human red cells
Authors: Lew VL, Etzion Z, Bookchin RM, daCosta R, Vaananen H, Sassaroli M, Eisinger J.
Journal: Biochim Biophys Acta (1993): 152



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