钙离子荧光探针Fluo-8L, AM
货号21097存储条件F/L
规格10x50 ug价格2340
Ex (nm)494Em (nm)517
分子量1078.95溶剂DMSO
产品详细介绍


简要概述

产品基本信息

货号:21097

产品名称:钙离子荧光探针Fluo-8L, AM 

规格:10x50ug

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:1078.95

溶剂:DMSO

激发波长(nm):494

发射波长(nm):517

 

产品介绍

        钙离子荧光探针Fluo-8L, AM 是美国AAT Bioquest生产的钙离子荧光探针,钙测量对于许多生物学研究至关重要。在结合Ca2 +后显示光谱响应的荧光探针使研究人员能够使用荧光显微镜,流式细胞仪,荧光光谱和荧光酶标仪来研究细胞内游离Ca2 +浓度的变化。在可见光激发钙指示剂中,Fluo-3和Fluo-4最常用。但是,Fluo-3 AM和Fluo-4 AM在酯酶水解后在活细胞中仅适度发荧光,并且需要苛刻的细胞加载条件才能最大化其细胞钙反应。开发Fluo-8®染料可改善细胞负载和钙响应,同时保持便捷的Fluo-3和Fluo-4光谱波长(在〜490 nm处具有最大激发和在〜520 nm处具有最大发射)。Fluo-8®AM仅需要室温,而Fluo-3 AM和Fluo-4 AM需要37?C的电池负载。此外,Fluo-8®的亮度是Fluo-4 AM的2倍,是Fluo-3 AM的4倍。AAT Bioquest提供了一套我们的出色Fluo-8®试剂,具有不同的钙结合亲和力(Fluo-8®:Kd = 389 nM; Fluo-8H:Kd = 232 nM; Fluo-8L:Kd = 1.86 µM; Fluo-8FF :Kd = 10 µM)。我们还提供多种包装尺寸以满足您的特殊需求,例如1毫克;10x50 µg;20x50 µg;HTS包装,不收取额外包装费用。Kd = 232 nM;Fluo-8L:Kd = 1.86 µM;Fluo-8FF:Kd = 10 µM)。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的钙离子荧光探针Fluo-8L, AM 。 

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点击查看实验方案

 

参考文献

2-OMe-lysophosphatidylcholine analogues are GPR119 ligands and activate insulin secretion from βTC-3 pancreatic cells: Evaluation of structure-dependent biological activity
Authors: Anna Drzazga, Agata Sowińska, Agnieszka Krzemińska, Andrzej Okruszek, Piotr Paneth, Maria Koziolkiewicz, Edyta Gendaszewska-Darmach
Journal: Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids (2017)

A new electro-optical approach for conductance measurement: an assay for the study of drugs acting on ligand-gated ion channels
Authors: A Menegon, S Pitassi, N Mazzocchi, L Redaelli, R Rizzetto, JF Rolland, C Poli, M Imberti, A Lanati, F Grohovaz
Journal: Scientific Reports (2017)

Altered spontaneous calcium signaling of in situ chondrocytes in human osteoarthritic cartilage
Authors: Xiaoyuan Gong, Wenbin Xie, Bin Wang, Lingchuan Gu, Fuyou Wang, Xiang Ren, Cheng Chen, Liu Yang
Journal: Scientific reports (2017): 17093

Analysis of Ca2+ response of osteocyte network by three-dimensional time-lapse imaging in living bone
Authors: Tomoyo Tanaka, Mitsuhiro Hoshijima, Junko Sunaga, Takashi Nishida, Mana Hashimoto, Naoya Odagaki, Ryuta Osumi, Taiji Aadachi, Hiroshi Kamioka
Journal: Journal of Bone and Mineral Metabolism (2017): 1--10

Aryl-and alkyl-phosphorus-containing flame retardants induced mitochondrial impairment and cell death in Chinese hamster ovary (CHO-k1) cells
Authors: Chao Huang, Na Li, Shengwu Yuan, Xiaoya Ji, Mei Ma, Kaifeng Rao, Zijian Wang
Journal: Environmental Pollution (2017): 775--786

Bystander effects elicited by single-cell photo-oxidative blue-light stimulation in retinal pigment epithelium cell networks
Authors: Masaaki Ishii, Bärbel Rohrer
Journal: Cell Death Discovery (2017): 16071

Ca 2+ signals initiate at immobile IP 3 receptors adjacent to ER-plasma membrane junctions
Authors: Nagendra Babu Thillaiappan, Alap P Chavda, Stephen C Tovey, David L Prole, Colin W Taylor
Journal: Nature Communications (2017): 1505

Cells smell on a CMOS: A portable odorant detection system using cell-laden collagen pillars
Authors: Yusuke Hirata, Yuya Morimoto, Eunryel Nam, Shotaro Yoshida, Shoji Takeuchi
Journal: (2017): 13--16

Ex vivo replication of phenotypic functions of osteocytes through biomimetic 3D bone tissue construction
Authors: Qiaoling Sun, Saba Choudhary, Ciaran Mannion, Yair Kissin, Jenny Zilberberg, Woo Y Lee
Journal: Bone (2017)

High Glucose Enhances Isoflurane-Induced Neurotoxicity by Regulating TRPC-Dependent Calcium Influx
Authors: ZhongJie Liu, ChangQing Ma, Wei Zhao, QingGuo Zhang, Rui Xu, HongFei Zhang, HongYi Lei, ShiYuan Xu
Journal: Neurochemical Research (2017): 1--14

 

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说明书
钙离子荧光探针Fluo-8L, AM .pdf