钙离子荧光探针Cal-520FF, AM
货号21142存储条件F/L
规格1 mg价格5940
Ex (nm)492Em (nm)514
分子量1138.92溶剂DMSO
产品详细介绍


简要概述

产品基本信息

货号:21142

产品名称:钙离子荧光探针Cal-520FF, AM

规格:1mg

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:1138.92

溶剂:DMSO

激发波长(nm):492

发射波长(nm):514

 

产品介绍

        钙离子荧光探针Cal-520FF, AM是美国AAT Bioquest生产的钙离子荧光探针,Cal-520®提供了一种功能强大的基于荧光的均质检测工具,用于检测细胞内钙的动员。Cal-520®AM是一种新型的荧光钙敏感染料,与现有的绿色钙指示剂(如Fluo-3 AM和Fluo-4 AM)相比,信噪比和细胞内滞留性显着提高。表达通过钙信号的感兴趣的GPCR或钙通道的细胞可以预装可穿过细胞膜的Cal-520®AM。一旦进入细胞内,Cal-520 AM的亲脂性封闭基团就会被酯酶裂解,从而产生带负电荷的荧光染料,并留在细胞内部。与钙结合后,其荧光大大增强。当细胞被激动剂刺激时,该受体发出信号释放细胞内钙,可以显着提高Cal-520®的荧光。Cal-520®AM具有长波长,高灵敏度和> 100倍荧光增强的特性,使其成为测量细胞钙的理想指示剂。高信噪比和更好的细胞内滞留性使Cal-520®钙测定成为评估GPCR和钙通道靶标以及筛选其激动剂和拮抗剂的强大工具。与其他Cal-520®指标相比,Cal-520FF 对钙的亲和力最低,Kd〜10 uM。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的钙离子荧光探针Cal-520FF, AM。 

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参考文献

A Critical Period for the Rapid Modification of Synaptic Properties at the VPm Relay Synapse
Authors: Libiao Pan, Junhua Yang, Qian Yang, Xiaomeng Wang, Liya Zhu, Yali Liu, Huifang Lou, Chou Xu, Ying Shen, Hao Wang
Journal: Frontiers in molecular neuroscience (2017)

Advances in Two-Photon Scanning and Scanless Microscopy Technologies for Functional Neural Circuit Imaging
Authors: Simon R Schultz, Caroline S Copeland, Amanda J Foust, Peter Quicke, Renaud Schuck
Journal: Proceedings of the IEEE (2017): 139--157

Bidirectional communication between sensory neurons and osteoblasts in an in vitro co-culture system
Authors: Daisuke Kodama, Takao Hirai, Hisataka Kondo, Kazunori Hamamura, Akifumi Togari
Journal: FEBS Letters (2017)

Ca 2+ signals initiate at immobile IP 3 receptors adjacent to ER-plasma membrane junctions
Authors: Nagendra Babu Thillaiappan, Alap P Chavda, Stephen C Tovey, David L Prole, Colin W Taylor
Journal: Nature Communications (2017): 1505

Calcium Imaging Across Large Areas of Intact Vascular Endothelium Reveals Stimulus-Specific Sensory Cells
Authors: Matthew D Lee, Calum Wilson, John G McCarron
Journal: The FASEB Journal (2017): 1005--8

Calcium transient assays for compound screening with human iPSC-derived cardiomyocytes: Evaluating new tools
Authors: Neil J Daily, Radleigh Santos, Joseph Vecchi, Pinar Kemanli, Tetsuro Wakatsuki
Journal: Journal of evolving stem cell research (2017): 1

Characterization of postsynaptic calcium signals in the pyramidal neurons of anterior cingulate cortex
Authors: Xu-Hui Li, Qian Song, Tao Chen, Min Zhuo
Journal: Molecular Pain (2017): 1744806917719847

Direct measurement of TRPV4 and PIEZO1 activity reveals multiple mechanotransduction pathways in chondrocytes
Authors: M Rocio Servin-Vences, Mirko Moroni, Gary R Lewin, Kate Poole
Journal: eLife (2017): e21074

Expression of the GluA2 subunit of glutamate receptors is required for the normal dendritic differentiation of cerebellar Purkinje cells
Authors: Masahiko Tanaka, Tomomi Senda, Naohide Hirashima
Journal: Neuroscience Letters (2017)

Extensive Ca 2+ leak through K4750Q cardiac ryanodine receptors caused by cytosolic and luminal Ca 2+ hypersensitivity
Authors: Akira Uehara, Takashi Murayama, Midori Yasukochi, Michael Fill, Minoru Horie, Toru Okamoto, Yoshiharu Matsuura, Kiyoko Uehara, Takahiro Fujimoto, Takashi Sakurai
Journal: The Journal of general physiology (2017): 199--218

 

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说明书
钙离子荧光探针Cal-520FF, AM.pdf