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台盼蓝钠盐 0.1M水溶液

英文名称:Trypan UltraBlue™, sodium salt *0.1 M aqueous solution*
产品参数
Ex (nm)-Em (nm)-
分子量~1000溶剂Water
存储条件在2-8度冷藏保存, 避免光照
产品概述

产品基本信息

产品名称:台盼蓝钠盐 0.1M水溶液 

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:~1000

溶剂:水

激发波长(nm):618

发射波长(nm):N/A

 

产品介绍

台盼UltraBlue 在细胞渗透性方面与台盼蓝相似。活细胞不可渗透。与台盼蓝相比,台盼UltraBlue对细胞的毒性较小,特别是对细胞表面受体的影响小。我们的Trypan UltraBlue 钠盐经过高度纯化。我们的Trypan UltraBlue 也可用于防止荧光染料(如FDA,罗丹明123,JC-1,TMRE,TMRM,Indo-1 AM,Fura-2 AM,钙黄绿素AM,Fluo-3 AM,Fluo-4 AM) ,Quest Fluo-8 AM和Rhod-4 AM)从细胞中泄漏出来。它可能抑制药物外排泵的活性,因为它含有如下所示的丙磺舒样部分。与丙磺舒相比,它是中性的,在水中高度可溶,并且使用方便。其细胞机制仍在调查中。我们的Trypan UltraBlue 经过高度纯化,多可使用0。5 mM,具有小的细胞毒性。我们推荐的浓度为0.25 mM。

点击查看光谱

 

参考文献

A prospective study on trypan blue capsule staining under air vs under viscoelastic
Authors: Wong VW, Lai TY, Lee GK, Lam PT, Lam DS.
Journal: Eye (2006): 820

Effect of trypan blue staining on the density and viability of lens epithelial cells in white cataract
Authors: Nanavaty MA, Johar K, Sivasankaran MA, Vasavada AR, Praveen MR, Zetterstrom C.
Journal: J Cataract Refract Surg (2006): 1483

Hypothermia of 8 degrees C protects cultured retinal pigment epithelial cells and retinal ganglion cells against trypan blue toxicity
Authors: Kunikata H, Abe T, Murata H, Sagara Y, Wakusawa R, Sato H, Yoshida M, Fuse N, Tamai M.
Journal: Am J Ophthalmol (2006): 754

Establishment and optimization of a flow cytometric method for evaluation of viability of CD34+ cells after cryopreservation and comparison with trypan blue exclusion staining
Authors: Humpe A, Beck C, Schoch R, Kneba M, Horst HA.
Journal: Transfusion (2005): 1208

Neurotoxic effects of trypan blue on rat retinal ganglion cells
Authors: Jin Y, Uchida S, Yanagi Y, Aihara M, Araie M.
Journal: Exp Eye Res (2005): 395

Photodynamic actions of indocyanine green and trypan blue on human lens epithelial cells in vitro
Authors: Melendez RF, Kumar N, Maswadi SM, Zaslow K, Glickmank RD.
Journal: Am J Ophthalmol (2005): 132

Safety of trypan blue 1% and indocyanine green 0.5% in assisting visualization of anterior capsule during phacoemulsification in mature cataract
Authors: Chung CF, Liang CC, Lai JS, Lo ES, Lam DS.
Journal: J Cataract Refract Surg (2005): 938

Toxic effects of indocyanine green, infracyanine green, and trypan blue on the human retinal pigmented epithelium
Authors: Kodjikian L, Richter T, Halberstadt M, Beby F, Flueckiger F, Boehnke M, Garweg JG.
Journal: Graefes Arch Clin Exp Ophthalmol (2005): 917

Trypan blue exclusion principle in the evaluation of fibroblast attachment in vitro using V79 cells on the conditioned root surface
Authors: Chandra RV, Bhat KM, Jagetia GC.
Journal: Quintessence Int (2005): 702

Trypan blue identifies antimetabolite treatment area in trabeculectomy
Authors: Healey PR, Crowston JG.
Journal: Br J Ophthalmol (2005): 1152

 

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