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3-氰基-7-羟基香豆素 CAS 19088-73-4

英文名称:3-Cyano-7-hydroxycoumarin *CAS 19088-73-4*
产品参数
Ex (nm)406Em (nm)450
分子量187.15溶剂DMSO
存储条件在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:3-氰基-7-羟基香豆素荧光参照标准

CAS:19088-73-4

储存条件:-20℃干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:187.15

外观:黄色固体粉末

溶剂:DMSO

激发波长(nm):406

发射波长(nm):450

稀释溶液:它可以在pH 7.4-7.5的磷酸盐或Tris缓冲液中稀释。

 

产品介绍

3-氰基-7-羟基香豆素用作3-氰基-7-羟基香豆素基酶底物的校准标准。它具有pH依赖性荧光。

点击查看光谱

 

适用仪器

荧光酶标仪  
Ex: 408nm
Em: 450nm
cutoff: 420nm
推荐孔板: 纯黑色孔板

 

溶解方案(溶剂以说明书为准)

  0.1 mg 0.5 mg 1 mg 5 mg 10 mg
1 mM 534.331 µL 2.672 mL 5.343 mL 26.717 mL 53.433 mL
5 mM 106.866 µL 534.331 µL 1.069 mL 5.343 mL 10.687 mL
10 mM 53.433 µL 267.165 µL 534.331 µL 2.672 mL 5.343 mL

 

参考文献

Characterization of asymmetric fluorogenic phosphonates as probes for developing organophosphorus hydrolases with broader stereoselectivity
Authors: Amitai G, Adani R, Limanovich O, Teitlboim S, Yishay S, Tveria L, Yacov G, Meshulam H, Raveh L.
Journal: Chem Biol Interact (2008): 249
Asymmetric fluorogenic organophosphates for the development of active organophosphate hydrolases with reversed stereoselectivity
Authors: Amitai G, Adani R, Yacov G, Yishay S, Teitlboim S, Tveria L, Limanovich O, Kushnir M, Meshulam H.
Journal: Toxicology (2007): 187
Rapid determination of enzyme activities of recombinant human cytochromes P450, human liver microsomes and hepatocytes
Authors: Ghosal A, Hapangama N, Yuan Y, Lu X, Horne D, Patrick JE, Zbaida S.
Journal: Biopharm Drug Dispos (2003): 375