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Buccutite FOL,马来酰亚胺 [FOLM]

英文名称:Buccutite™ FOL, maleimide [FOLM]
Buccutite FOL,马来酰亚胺 [FOLM]
价格 2581
产品规格
2 umoles

产品货号
产品参数
Ex (nm)-Em (nm)-
分子量523.63溶剂DMSO
存储条件在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:Buccutite FOL,马来酰亚胺 [FOLM]

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

外观:固体

分子量:523.63

溶剂:DMSO

 

产品介绍

Buccutite 交联技术提供了方便、有效的交联方法,以高共轭产率连接两个生物分子。该方法使用一对交联剂:Buccutite MTA 和 Buccutite FOL。 MTA 被添加到一个分子中,而 FOL 被添加到另一个分子中。通过在中性条件下混合 Molecule-1-Buccutite MTA 和 Molecule-2-Buccutite FOL 来引发交联反应。Buccutite FOL 马来酰亚胺 (FOLM) 的使用方式与广泛用于交联蛋白质的 SMCC 相同。 FOLM 的一端(通过马来酰亚胺)与还原抗体(通过 TCEP 或 DTT)中发现的半胱氨酸硫醇 (-SH) 反应,以产生 FOL 修饰的还原抗体。 Buccutite 交联反应在极其温和和中性的条件下发生,不需要任何催化剂,而 SMCC 交联需要高浓度的蛋白质。此外,SMCC 修饰的蛋白质极不稳定,通常会自我反应,因为蛋白质通常同时含有胺和硫醇基团,这会导致大量的同交联。Buccutite 交联反应在极其温和和中性的条件下发生,无需任何催化剂,它比SMCC更加稳定。

 

参考文献

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