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7-羟基-4-甲基香豆素 CAS 90-33-5

英文名称:7-Hydroxy-4-methylcoumarin [4-Methylumbelliferone] *Fluorescence reference standard*
产品参数
Ex (nm)360Em (nm)448
分子量176.17溶剂DMSO
存储条件在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:7-羟基-4-甲基香豆素*荧光参照标准*

CAS:90-33-5

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:176.17

外观:灰白色粉末

溶剂:DMSO

激发波长(nm):360

发射波长(nm):448

 

产品介绍

        该化合物用作基于7-羟基-4-甲基香豆素的酶底物的校准标准。 它具有pH依赖性荧光。

点击查看光谱

 

适用仪器

荧光酶标仪  
Ex: 586nm
Em: 605nm
Cutoff: 590nm
推荐孔板: 纯黑色孔板
滤波片组: TRITC

 

溶解方案(溶剂以说明书为准)

  0.1 mg 0.5 mg 1 mg 5 mg 10 mg
1 mM 567.634 µL 2.838 mL 5.676 mL 28.382 mL 56.763 mL
5 mM 113.527 µL 567.634 µL 1.135 mL 5.676 mL 11.353 mL
10 mM 56.763 µL 283.817 µL 567.634 µL 2.838 mL 5.676 mL

 

参考文献

Excited state properties of 7-hydroxy-4-methylcoumarin in the gas phase and in solution. A theoretical study
Authors: Georgieva I, Trendafilova N, Aquino A, Lischka H.
Journal: J Phys Chem A Mol Spectrosc Kinet Environ Gen Theory (2005): 11860
Novel thiocoumarins as inhibitors of TNF-alpha induced ICAM-1 expression on human umbilical vein endothelial cells (HUVECs) and microsomal lipid peroxidation
Authors: Kumar S, Singh BK, Kalra N, Kumar V, Kumar A, Prasad AK, Raj HG, Parmar VS, Ghosh B.
Journal: Bioorg Med Chem (2005): 1605
Stability of the complexes of some lanthanides with coumarin derivatives. I. Cerium(III)-4-methyl-7-hydroxycoumarin
Authors: Kostova IP, Manolov, II, Radulova MK.
Journal: Acta Pharm (2004): 37
Measurement of enzyme kinetics using microscale steady-state kinetic analysis
Authors: Gleason NJ, Carbeck JD.
Journal: Langmuir (2004): 6374
Structural requirements of hydroxylated coumarins for in vitro anti-Helicobacter pylori activity
Authors: Kawase M, Tanaka T, Sohara Y, Tani S, Sakagami H, Hauer H, Chatterjee SS.
Journal: In Vivo (2003): 509
Crystal structure of 7,8-dihydroxy-4-methylcoumarin
Authors: Kurosaki H, Sharma RK, Otsuka M, Goto M.
Journal: Anal Sci (2003): 647
Modifying rates of reductive elimination of leaving groups from indolequinone prodrugs: a key factor in controlling hypoxia-selective drug release
Authors: Everett SA, Swann E, Naylor MA, Stratford MR, Patel KB, Tian N, Newman RG, Vojnovic B, Moody CJ, Wardman P.
Journal: Biochem Pharmacol (2002): 1629
Effects of 18 alpha-glycyrrhizic acid on rat liver cytochrome P450 isoenzymes and phase II transferase
Authors: Yang J, Peng RX, Kong R, Yu JP.
Journal: Yao Xue Xue Bao (2001): 321
Synthesis and evaluation of novel fluorogenic substrates for the detection of bacterial beta-galactosidase
Authors: Chilvers KF, Perry JD, James AL, Reed RH.
Journal: J Appl Microbiol (2001): 1118
Antioxidative and prooxidative effects of coumarin derivatives on free radical initiated and photosensitized peroxidation of human low-density lipoprotein
Authors: Liu ZQ, Yu W, Liu ZL.
Journal: Chem Phys Lipids (1999): 125