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Buccutite 聚HRP抗体偶联试剂盒

英文名称:Buccutite™ Poly-HRP Antibody Conjugation Kit
Buccutite 聚HRP抗体偶联试剂盒
价格 5237
产品规格
1x50 ug Labeling

产品货号
产品参数
Ex (nm)-Em (nm)-
分子量-溶剂Water
存储条件在2-8度冷藏保存, 避免光照
产品概述

在样品有限或目标分子含量极低的免疫测定中,聚-HRP-抗体结合物被认为具有高的敏感性。 Buccutite 聚HRP抗体偶联试剂盒旨在快速制备聚HRP偶联抗体。该试剂盒提供了可通过我们专有的Buccutite FOL预的poly-HRP探针。用Buccutite MTA(试剂盒中提供)靶向抗体,以得到MTA的抗体。 MTA的抗体与FOL的多HRP的简单混合即可得到所需的多HRP标记的抗体偶联物。该反应在极温和的条件下进行而无需催化剂。用Buccutite Poly-HRP抗体偶联试剂盒制备的poly-HRP抗体偶联物与ELISA,western blotting,免疫组化(IHC)分析中使用的发色,荧光和化学发光HRP底物兼容。它也可以与TSA或我们的Styramide 荧光HRP底物一起使用,以超灵敏地检测低丰度生物靶标。

 

图示

图1.使用Buccutite Poly-HRP抗体偶联试剂盒(Cat#5518或Cat#5519)制备的polyHRP-山羊抗小鼠IgG偶联物生成直接ELISA曲线。将3倍系列稀释的小鼠IgG包被在96孔板上,并使用标准ELISA方法检测100uL GAM IgG-polyHRP缀合物(100ng / ml)。用TMB底物溶液(Cat#11003)孵育5分钟检测固定的小鼠IgG,并在450 nm处读数。
蓝色:5518或5519试剂盒
红色:GXM IgG PolyHRP(供应商A)
绿色:GXM IgG-HRP(供应商B)

 

参考文献

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