Buccutite 聚HRP抗体偶联试剂盒

货号5518存储条件 在2-8度冷藏保存, 避免光照
规格1x50 ug Labeling价格4740
Ex (nm)Em (nm)
分子量溶剂
产品详细介绍


简要概述

在样品有限或目标分子含量极低的免疫测定中,聚-HRP-抗体结合物被认为具有最高的敏感性。 Buccutite 聚HRP抗体偶联试剂盒旨在快速制备聚HRP偶联抗体。该试剂盒提供了可通过我们专有的Buccutite FOL预激活的poly-HRP探针。用Buccutite MTA(试剂盒中提供)激活靶向抗体,以得到MTA激活的抗体。 MTA激活的抗体与FOL激活的多HRP的简单混合即可得到所需的多HRP标记的抗体偶联物。该反应在极温和的条件下进行而无需催化剂。用Buccutite Poly-HRP抗体偶联试剂盒制备的poly-HRP抗体偶联物与ELISA,western blotting,免疫组化(IHC)分析中使用的发色,荧光和化学发光HRP底物兼容。它也可以与TSA或我们的Styramide 荧光HRP底物一起使用,以超灵敏地检测低丰度生物靶标。

 

图示

图1.使用Buccutite Poly-HRP抗体偶联试剂盒(Cat#5518或Cat#5519)制备的polyHRP-山羊抗小鼠IgG偶联物生成直接ELISA曲线。将3倍系列稀释的小鼠IgG包被在96孔板上,并使用标准ELISA方法检测100uL GAM IgG-polyHRP缀合物(100ng / ml)。用TMB底物溶液(Cat#11003)孵育5分钟检测固定的小鼠IgG,并在450 nm处读数。
蓝色:5518或5519试剂盒
红色:GXM IgG PolyHRP(供应商A)
绿色:GXM IgG-HRP(供应商B)

 

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