Cell Meter 固定化细胞和组织TUNEL细胞凋亡测定试剂盒*绿色荧光*

货号22851存储条件 在零下15度以下保存, 避免光照
规格25 Tests价格4884
Ex (nm)498Em (nm)517
分子量溶剂
产品详细介绍


简要概述

产品基本信息

货号:22851

产品名称:Cell Meter 固定化细胞和组织TUNEL细胞凋亡测定试剂盒*绿色荧光*

规格:25 Tests

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

Ex(nm):498

Em(nm):517

 

适用仪器


流式细胞仪  
激发: 488nm激光
发射: 530/30nm滤波片
通道: FITC通道
荧光显微镜  
激发: FITC滤波片组
发射: FITC滤波片组
推荐孔板: 黑色透明

 

产品介绍

Cell Meter 固定化细胞和组织TUNEL细胞凋亡检测试剂盒提供了一种强大荧光检测工具,可方便地检测由凋亡引起的DNA片段化。该检测方法是一种非放射性,简单,准确和快速的方法,可通过对DNA片段进行成像来检测固定细胞和组织中的细胞凋亡。TUNEL分析使用末端脱氧核苷酸转移酶(TdT)催化在片段化DNA的3'-羟基末端掺入荧光素12-dUTP。通过荧光显微镜或流式细胞仪(用530/30 nm发射滤光片在488 nm激发)分析荧光素标记的DNA。该试剂盒可用于检测固定细胞和福尔马林固定的石蜡包埋的组织切片的凋亡。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的Cell Meter 固定化细胞和组织TUNEL细胞凋亡测定试剂盒。 

 

图示

 

图1.固定HeLa细胞,并在37°C下用或不使用DNAse处理60分钟。然后将细胞用Cell Meter TUNEL细胞凋亡检测试剂盒染色。DNA链断裂在DNAse处理的细胞中显示出强烈的荧光染色。使用FITC滤光片组通过荧光显微镜检测信号。

 

 

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Cell Meter 固定化细胞和组织TUNEL细胞凋亡测定试剂盒*绿色荧光*.pdf