Cell Meter 荧光法细胞周期检测试剂盒 405nm激发

货号22845存储条件f/l
规格100 Tests价格2460
Ex (nm)Em (nm)
分子量溶剂
产品详细介绍


简要概述

细胞周期具有四个连续阶段:G0 / G1,S,G2和M。在细胞通过细胞周期的过程中,其DNA在S(合成)阶段复制,并在M(有丝分裂)阶段在两个子细胞之间平均分配。这两个阶段由两个间隙阶段分开:G0 / G1和G2。这两个间隙相为细胞提供了生长时间,并使它们的蛋白质和细胞器的质量增加了一倍。在进入下一阶段的细胞周期之前,细胞还将它们用于检测内部和外部条件。细胞通过细胞周期的传递受到许多不同调节蛋白的控制。该特定试剂盒旨在通过在活细胞中使用我们专有的Nuclear Violet 检测细胞周期的进程和增殖。可以通过流式细胞术确定给定样品中处于G0 / G1,S和G2 / M期的细胞以及亚G1期处于凋亡之前的细胞的百分比。可以用流式细胞仪(PacificBlue®通道)检测用Nuclear Violet 染色的细胞。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的Cell Meter 荧光法细胞周期检测试剂盒。



产品说明书

样品实验方案

简要概述

  1. 用5×105至1×106细胞/ mL的密度制备含测试化合物的细胞
  2. 在0.5 mL细胞溶液中加入1 µL 500X Nuclear Violet
  3. 在室温下孵育30-60分钟
  4. 使用405nm紫色光激发流式细胞仪进行分析

 

实验步骤

1.对于每个样品,请在0.5 mL的温暖培养基或自备缓冲液中以5×105至1×106细胞/ mL的密度制备细胞。注意:应单独评估每种细胞系,以确定诱导凋亡的最佳细胞密度。

2.用测试化合物处理细胞,以诱导凋亡或其他细胞周期功能。

3.向含有生长培养基的细胞中加入1µL 500X Nuclear Violet (组分A),并在37°C,5%CO2的培养箱中孵育细胞30至60分钟。注意:对于贴壁细胞,用0.5mM EDTA轻轻提起细胞以保持细胞完整,并在用Nuclear Violet 孵育之前用含血清的培养基洗涤一次。注意:适当的孵育时间取决于所用的单个细胞类型和细胞浓度。优化每个实验的孵育时间。注意:DNA染色前无需固定细胞,因为Nuclear Violet 具有细胞渗透性。

4.可选:以1000 rpm离心细胞4分钟,然后将细胞重悬于0.5 mL检测缓冲液(组分B)或自备缓冲液中。

5.使用405 nm紫色激光(Ex / Em = 405/445 nm)通过流式细胞仪检测荧光强度。

 

参考文献

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Direct control of cell cycle gene expression by proto-oncogene product ACTR, and its autoregulation underlies its transforming activity
Authors: Louie MC, Revenko AS, Zou JX, Yao J, Chen HW.
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Authors: Easwaran HP, Leonhardt H, Cardoso MC.
Journal: Cell Cycle (2005): 453

Dynamic relocalization of hOGG1 during the cell cycle is disrupted in cells harbouring the hOGG1-Cys326 polymorphic variant
Authors: Luna L, Rolseth V, Hildrestr and GA, Otterlei M, Dantzer F, Bjoras M, Seeberg E.
Journal: Nucleic Acids Res (2005): 1813

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Cell cycle regulation of the murine 8-oxoguanine DNA glycosylase (mOGG1): mOGG1 associates with microtubules during interphase and mitosis
Authors: Conlon KA, Zharkov DO, Berrios M.
Journal: DNA Repair (Amst) (2004): 1601

Description of a flow cytometry approach based on SYBR-14 staining for the assessment of DNA content, cell cycle analysis, and sorting of living normal and neoplastic cells
Authors: Nunez R, Garay N, Villafane C, Bruno A, Lindgren V.
Journal: Exp Mol Pathol (2004): 29

Differential roles of STAT1alpha and STAT1beta in fludarabine-induced cell cycle arrest and apoptosis in human B cells
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Journal: Blood (2004): 2475



说明书
Cell Meter 荧光法细胞周期检测试剂盒 405nm激发 .pdf