D-荧光素半乳糖苷 CAS 131474-38-9
英文名称：D-Luciferin galactoside *CAS 131474-38-9*
产品参数
| Ex (nm) | 385 | Em (nm) | 529 |
| 分子量 | 442.46 | 溶剂 | DMSO |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品概述
产品货期
咨询
产品优势
1.双功能底物，它可同步检测β-半乳糖苷酶（LacZ基因）和萤光素酶活性
2.灵敏度高
适用范围
可同步检测β-半乳糖苷酶（LacZ基因）和萤光素酶活性
产品介绍
D-萤光素半乳糖苷的6-O位连接有β-半乳糖苷基团，该结构阻碍萤火虫萤光素酶对其识别。然而，β-半乳糖苷酶可有效切除半乳糖基团，生成的D-萤光素能够被萤光素酶正常识别。该化合物是一种高灵敏度化学发光底物，适用于均相检测或细胞裂解液样本中半乳糖苷酶活性的测量（需联用萤光素酶及其辅因子）。
作为双功能底物，它可同步检测β-半乳糖苷酶（LacZ基因）和萤光素酶活性，且具有极高灵敏度。在表达萤火虫萤光素酶的模型系统中，酶解释放的萤光素可参与萤火虫萤光素酶反应产生发光信号。该底物亦适用于体内生物发光成像研究。
Luc的Ex/Em及其他相关内容链接：Spectral properties of luciferin and EGFP. A: Normalized excitation and... | Download Scientific Diagram (researchgate.net)
试剂应用文献
Extracellular vesicle surface display of $\alpha$PD-L1 and $\alpha$CD3 antibodies via engineered late domain-based scaffold to activate T-cell anti-tumor immunity
Authors: Chen, Rui and Kang, Ziqin and Li, Wenhao and Xu, Tianshu and Wang, Yongqiang and Jiang, Qiming and Wang, Yuepeng and Huang, Zixian and Xu, Xiaoding and Huang, Zhiquan
Journal: Journal of Extracellular Vesicles (2024): e12490
Authors: Chen, Rui and Kang, Ziqin and Li, Wenhao and Xu, Tianshu and Wang, Yongqiang and Jiang, Qiming and Wang, Yuepeng and Huang, Zixian and Xu, Xiaoding and Huang, Zhiquan
Journal: Journal of Extracellular Vesicles (2024): e12490
Bmal1 integrates circadian function and temperature sensing in the suprachiasmatic nucleus
Authors: Hoekstra, Marieke MB and Ness, Natalie and Badia-Soteras, Aina and Brancaccio, Marco
Journal: Proceedings of the National Academy of Sciences (2024): e2316646121
Authors: Hoekstra, Marieke MB and Ness, Natalie and Badia-Soteras, Aina and Brancaccio, Marco
Journal: Proceedings of the National Academy of Sciences (2024): e2316646121
