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1mM DEAC-dUTP溶液

英文名称:DEAC-dUTP *1 mM in Tris Buffer (pH 7.5)*
产品参数
Ex (nm)411Em (nm)472
分子量945.58溶剂Water
存储条件在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:1mM DEAC-dUTP溶液

储存条件:-15℃避光干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:945.58

溶剂:水

激发波长(nm):408

发射波长(nm):470

 

产品介绍

染料修饰的脱氧尿苷5'-三磷酸酯(例如氨基烯丙基-dUTP)可用于通过常规酶促掺入方法(例如逆转录,缺口翻译,随机引物标记或PCR)生产含染料的DNA。这种酶促荧光标记方法广泛用于FISH探针和基于微阵列的实验。该香豆素-dUTP偶联物可与Spectrum Aqua 滤光片组合一起用作蓝色荧光色(Spectrum Aqua 是Vysis的商标)。

点击查看光谱

 

参考文献

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Simple method for preparation of fluor/hapten-labeled dUTP
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Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
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Journal: Biochemistry (2000): 13405

Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
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Journal: Anal Biochem (1993): 229

A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
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Journal: J Virol Methods (1992): 145

Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
Authors: Dawson BA, Herman T, Haas AL, Lough J.
Journal: J Cell Biochem (1991): 166

Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2'-deoxyuridine-5'-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
Authors: Muhlegger K, Huber E, von der Eltz H, Ruger R, Kessler C.
Journal: Biol Chem Hoppe Seyler (1990): 953