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荧光素-12-dUTP *1 mM,溶于 Tris 缓冲液 (pH 7.5)* *CAS 214154-36-6*

英文名称:Fluorescein-12-dUTP *1 mM in Tris Buffer (pH 7.5)* *CAS 214154-36-6*
产品参数
Ex (nm)498Em (nm)517
分子量1060.62溶剂Water
存储条件在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:荧光素-12-dUTP *1 mM,溶于 Tris 缓冲液 (pH 7.5)* *CAS 214154-36-6*

CAS:214154-36-6

储存条件:-80℃避光干燥

保质期:12个月

 

产品物理化学光谱特性

分子量:1060.62

外观:液体

溶剂:10mM Tris-HCl + 1mM EDTA (pH=7.5)

激发波长(nm):492

发射波长(nm):515

 

产品介绍

染料修饰的脱氧尿苷5'-三磷酸(例如氨基烯丙基-dUTP)可用于通过常规酶掺入方法如逆转录,切口平移,随机引物标记或PCR产生含染料的DNA。该酶荧光标记方法广泛用于FISH探针和基于微阵列的实验。该荧光素-dUTP缀合物可用作具有Spectrum Green™过滤器组的绿色荧光颜色(Spectrum Green ™是Vysis的商标)。

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参考文献

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Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking
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Journal: Biochemistry (2000): 13405

Quantitative analysis of polymerase chain reaction products using biotinylated dUTP incorporation
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A non-radioisotopic reverse transcriptase assay using biotin-11-deoxyuridinetriphosphate on primer-immobilized microtiter plates
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Journal: J Virol Methods (1992): 145

Affinity isolation of active murine erythroleukemia cell chromatin: uniform distribution of ubiquitinated histone H2A between active and inactive fractions
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Journal: J Cell Biochem (1991): 166

Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2'-deoxyuridine-5'-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin)
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Journal: Biol Chem Hoppe Seyler (1990): 953