FluoroQuest 防褪色固定介质（含DAPI）

具有DAPI的FluoroQuest 是一种水性固定介质，用于保留组织和细胞涂片的荧光。这种封固剂含有抗褪色剂以降低染料的光漂白速率，并以DAPI强化，DAPI是DNA的复染剂。用于原位杂交技术或需要荧光法进行DNA染色的其他方法。DAPI在360 nm处激发并在460 nm处发射，产生蓝色荧光。百萤生物是AAT Bioquest的中国代理商，为您提供优质的FluoroQuest 防褪色固定介质。 

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样品实验方案

以下是我们推荐的方案，仅提供指导。具体实验应根据您的特定需求进行修改。

1. 预热溶液：从冰箱中取出安装溶液。将瓶子加热至室温，避光。
2. 添加溶液：清除样品中多余的液体。向样品中加一滴固定溶液。如果样品在载玻片或组织培养皿上，请小心地将盖玻片放在液滴上，以免产生气泡。如果样品在盖玻片上，请将盖玻片倒在干净的玻璃载玻片上。去除多余的防褪色成分。
3. 准备用于成像的样品：固定溶液应孵育2小时至过夜。要长期存放，请用指甲油或塑料密封剂将盖玻片密封在玻片上。已安装的载玻片应在黑暗中于4°C存放，荧光成像将保持稳定数周，样品可以在安装后立即成像。 

图示

图1. 甲醛固定，石蜡包埋的人肺腺癌阳性组织的荧光IHC 。用兔抗EpCam抗体对人肺腺癌阳性组织切片进行染色，然后分别与iFluor 488 Styramide （Cat＃45020）染色的聚HRP标记的羊抗兔IgG二抗一起孵育。将组织固定在带有DAPI（Cat#20005）的FluoroQuest 抗褪色固定介质中。

参考文献

Photobleaching of organic fluorophores: quantitative characterization, mechanisms, protection.
Authors: Demchenko, Alexander P
Journal: Methods and applications in fluorescence (2020): 022001

The Relationship between Residual Amount of Sr and Morphology of Eutectic Si Phase in A356 Alloy.
Authors: Zhang, Wenda and Ma, Shixuan and Wei, Zhenhua and Bai, Peikang
Journal: Materials (Basel, Switzerland) (2019)

[Multi-color immunofluorescence microscopy in cultured cells].
Authors: Matsuzaki, Toshiyuki
Journal: Nihon yakurigaku zasshi. Folia pharmacologica Japonica (2019): 165-170

Using Phospho-Peptides Immobilized on Magnetic Beads for Absorption Control in Immunohistochemistry.
Authors: Schwartz, David and Grahek, Michael and He, Yingwei and Wang, Wei and Nguyen, Jennifer and Kalyuzhny, Alexander E
Journal: Methods in molecular biology (Clifton, N.J.) (2017): 219-227

A Power-Efficient Clustering Protocol for Coal Mine Face Monitoring with Wireless Sensor Networks Under Channel Fading Conditions.
Authors: Ren, Peng and Qian, Jiansheng
Journal: Sensors (Basel, Switzerland) (2016)

FAD-I, a Fusobacterium nucleatum Cell Wall-Associated Diacylated Lipoprotein That Mediates Human Beta Defensin 2 Induction through Toll-Like Receptor-1/2 (TLR-1/2) and TLR-2/6.
Authors: Bhattacharyya, Sanghamitra and Ghosh, Santosh K and Shokeen, Bhumika and Eapan, Betty and Lux, Renate and Kiselar, Janna and Nithianantham, Stanley and Young, Andrew and Pandiyan, Pushpa and McCormick, Thomas S and Weinberg, Aaron
Journal: Infection and immunity (2016): 1446-1456

Production of colourful pigments consisting of amorphous arrays of silica particles.
Authors: Yoshioka, Shinya and Takeoka, Yukikazu
Journal: Chemphyschem : a European journal of chemical physics and physical chemistry (2014): 2209-15

A dendritic single-molecule fluorescent probe that is monovalent, photostable and minimally blinking.
Authors: Yang, Si Kyung and Shi, Xinghua and Park, Seongjin and Ha, Taekjip and Zimmerman, Steven C
Journal: Nature chemistry (2013): 692-7

A comparison of fluorescent stains for the assessment of viability and metabolic activity of lactic acid bacteria.
Authors: Zotta, T and Guidone, A and Tremonte, P and Parente, E and Ricciardi, A
Journal: World journal of microbiology & biotechnology (2012): 919-27

Anti-fading media for live cell GFP imaging.
Authors: Bogdanov, Alexey M and Kudryavtseva, Elena I and Lukyanov, Konstantin A
Journal: PloS one (2012): e53004