iFluor 350 酪胺
英文名称：iFluor® 350 Tyramide
产品参数
| Ex (nm) | 345 | Em (nm) | 450 |
| 分子量 | 432.45 | 溶剂 | DMSO |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品概述
产品基本信息
产品名称：iFluor 350 酪胺
储存条件：-15℃避光防潮
保质期：12个月
注：200slides：一管试剂足够200个玻片使用；注2：200slides大约为100ug。
产品物理化学光谱特性
分子量：432.45
Ex(nm)：345
Em(nm)：450
量子产率(cm -1 M -1)：20000
消光系数：0.95
产品介绍
对于许多免疫组织化学（IHC）的应用，传统的酶扩增程序足以实现充分的抗原检测。然而，有几个因素限制了这些程序的敏感性和实用性。酪 胺信号放大技术（TSA）已被证明是一种特别通用和强大的酶放大技术，提高了检测灵敏度。TSA是基于HRP在低浓度过氧化氢存在下将标记的酪胺底物转化为氧化的、高活性的自由基的能力，该自由基可在HRP处或其附近共价结合酪氨酸残基。为了达到大限度的IHC检测，酪胺预先标记了一个荧光团。通过每个过氧化物酶标签上的多个酪胺底物的转换所产生的信号放大转化为对低丰度目标的超灵敏检测，以及使用少量抗体和杂交探针。在免疫组化应用中，TSA法的敏感性增强使原代抗体稀释度增加，以减少非特异性背景信号，并能克服由于固定程序不理想或靶蛋白表达水平低而导致的弱免疫标记。iFluor 350酪胺含有明亮的iFluor350，可以很容易地用标准DAPI滤波片组检测。它是Alexa Fluor®350酪胺（Alexa Fluor®是ThermoFisher的商标）或其他光谱类似的荧光酪胺共轭物或TSA试剂（如荧光素酪胺）的极好替代品。百萤生物是AAT Bioquest的中国代理商，为您提供优质的iFluor 350 酪胺。
参考文献
Ultrastructure of light-activated axons following optogenetic stimulation to produce late-phase long-term potentiation.
Authors: Kuwajima, Masaaki and Ostrovskaya, Olga I and Cao, Guan and Weisberg, Seth A and Harris, Kristen M and Zemelman, Boris V
Journal: PloS one (2020): e0226797
Authors: Kuwajima, Masaaki and Ostrovskaya, Olga I and Cao, Guan and Weisberg, Seth A and Harris, Kristen M and Zemelman, Boris V
Journal: PloS one (2020): e0226797
Intensive Immunofluorescence Staining Methods for Low Expression Protein: Detection of Intestinal Stem Cell Marker LGR5.
Authors: Yamazaki, Masaki and Kato, Atsuhiko and Zaitsu, Yoko and Watanabe, Takeshi and Iimori, Makoto and Funahashi, Shinichi and Kitao, Hiroyuki and Saeki, Hiroshi and Oki, Eiji and Suzuki, Masami
Journal: Acta histochemica et cytochemica (2015): 159-64
Authors: Yamazaki, Masaki and Kato, Atsuhiko and Zaitsu, Yoko and Watanabe, Takeshi and Iimori, Makoto and Funahashi, Shinichi and Kitao, Hiroyuki and Saeki, Hiroshi and Oki, Eiji and Suzuki, Masami
Journal: Acta histochemica et cytochemica (2015): 159-64
Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry.
Authors: Clutter, Matthew R and Heffner, Garrett C and Krutzik, Peter O and Sachen, Kacey L and Nolan, Garry P
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2010): 1020-31
Authors: Clutter, Matthew R and Heffner, Garrett C and Krutzik, Peter O and Sachen, Kacey L and Nolan, Garry P
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (2010): 1020-31
Methoxychlor and estradiol induce oxidative stress DNA damage in the mouse ovarian surface epithelium.
Authors: Symonds, Daniel A and Merchenthaler, Istvan and Flaws, Jodi A
Journal: Toxicological sciences : an official journal of the Society of Toxicology (2008): 182-7
Authors: Symonds, Daniel A and Merchenthaler, Istvan and Flaws, Jodi A
Journal: Toxicological sciences : an official journal of the Society of Toxicology (2008): 182-7
Genotyping of phenotypically defined cells in neoplasia: enhanced immunoFISH via tyramide signal amplification (TSA) segregates immunophenotypically-defined cell populations for gated genotyping.
Authors: Tubbs, Raymond R and Das, Kingshuk and Cook, James R and Pettay, James D and Roche, Patrick C and Grogan, Thomas
Journal: Journal of molecular histology (2007): 129-34
Authors: Tubbs, Raymond R and Das, Kingshuk and Cook, James R and Pettay, James D and Roche, Patrick C and Grogan, Thomas
Journal: Journal of molecular histology (2007): 129-34
A CARD-FISH protocol for the identification and enumeration of epiphytic bacteria on marine algae.
Authors: Tujula, Niina A and Holmström, Carola and Mussmann, Marc and Amann, Rudolf and Kjelleberg, Staffan and Crocetti, Gregory R
Journal: Journal of microbiological methods (2006): 604-7
Authors: Tujula, Niina A and Holmström, Carola and Mussmann, Marc and Amann, Rudolf and Kjelleberg, Staffan and Crocetti, Gregory R
Journal: Journal of microbiological methods (2006): 604-7
Novel oxidative self-anchoring fluorescent substrates for the histochemical localization of endogenous and immunobound peroxidase activity.
Authors: Krieg, Reimar and Halbhuber, Karl-Jürgen
Journal: Journal of molecular histology (2004): 471-87
Authors: Krieg, Reimar and Halbhuber, Karl-Jürgen
Journal: Journal of molecular histology (2004): 471-87
Simultaneous discrimination between 15 fish pathogens by using 16S ribosomal DNA PCR and DNA microarrays.
Authors: Warsen, Adelaide E and Krug, Melissa J and LaFrentz, Stacey and Stanek, Danielle R and Loge, Frank J and Call, Douglas R
Journal: Applied and environmental microbiology (2004): 4216-21
Authors: Warsen, Adelaide E and Krug, Melissa J and LaFrentz, Stacey and Stanek, Danielle R and Loge, Frank J and Call, Douglas R
Journal: Applied and environmental microbiology (2004): 4216-21
Detection of pathogenic Vibrio spp. in shellfish by using multiplex PCR and DNA microarrays.
Authors: Panicker, Gitika and Call, Douglas R and Krug, Melissa J and Bej, Asim K
Journal: Applied and environmental microbiology (2004): 7436-44
Authors: Panicker, Gitika and Call, Douglas R and Krug, Melissa J and Bej, Asim K
Journal: Applied and environmental microbiology (2004): 7436-44
Identifying antimicrobial resistance genes with DNA microarrays.
Authors: Call, Douglas R and Bakko, Marlene K and Krug, Melissa J and Roberts, Marilyn C
Journal: Antimicrobial agents and chemotherapy (2003): 3290-5
Authors: Call, Douglas R and Bakko, Marlene K and Krug, Melissa J and Roberts, Marilyn C
Journal: Antimicrobial agents and chemotherapy (2003): 3290-5
