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iFluor 488-dUTP * 1 mM的Tris缓冲液(pH 7.5)*

英文名称:iFluor®488-dUTP *1 mM in Tris Buffer (pH 7.5)*
iFluor 488-dUTP * 1 mM的Tris缓冲液(pH 7.5)*
价格 1259
产品规格
25 nmoles

产品货号
产品参数
Ex (nm)491Em (nm)516
分子量1152.83溶剂Water
存储条件在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:iFluor 488-dUTP

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:1152.83

溶剂:水

激发波长(nm):491

发射波长(nm):516

 

产品介绍

染料修饰的脱氧尿苷5'-三磷酸酯是通过常规酶掺入方法(例如反转录,缺口翻译,随机引物标记或PCR)生产染料标记DNA的常用方法之一。 这种酶促荧光标记方法广泛用于FISH探针和基于微阵列的实验。 该iFluor 488-dUTP缀合物与SpectrumGreen 滤光片组合一起用作绿色荧光(SpectrumGreen 是Vysis的商标)。 与常用的绿色探针(例如Fluorescein-12-dUTP)相比,iFluor 488提供了更明亮的信号,具有很高的光稳定性,并且不受pH值的影响。

点击查看光谱

 

参考文献

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Flow cytometric detection of beta-D-glucuronidase gene in wild-type bacterial cells using in-situ PCR.
Authors: Sachidanandham, Ramaiah and Gin, Karina Yew-Hoong
Journal: Biotechnology and bioengineering (2003): 127-33

Lack of nuclear apoptosis in cardiomyocytes and increased endothelin-1 levels in a rat heart model of myocardial stunning.
Authors: Klainguti, M and Aigner, S and Kilo, J and Eppenberger, H M and Mandinova, A and Aebi, U and Schaub, M C and Shaw, S G and Lüscher, T F and Atar, D
Journal: Basic research in cardiology (2000): 308-15

A method to monitor DNA transfer during transfection.
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Journal: AAPS pharmSci (1999): E6

Pulsatile shear stress leads to DNA fragmentation in human SH-SY5Y neuroblastoma cell line.
Authors: Triyoso, D H and Good, T A
Journal: The Journal of physiology (1999): 355-65

Detection of TNF alpha and Fas ligand mRNA within synovial mononuclear cells by fluorescence in-cell labeling PCR (FICL-PCR).
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Journal: Molecular biology reports (1998): 217-24

Targeted nucleotide exchange in the alkaline phosphatase gene of HuH-7 cells mediated by a chimeric RNA/DNA oligonucleotide.
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