荧光素酶 重组荧光素

货号12500存储条件f/l
规格1 mL价格1200
Ex (nm)Em (nm)
分子量 ~60 kDa 溶剂Water
产品详细介绍


简要概述

产品基本信息

货号:12500

产品名称:荧光素酶 重组荧光素

规格:1ml

储存条件:-15℃避光防潮

保质期:24个月

 

产品介绍

荧光素酶 重组荧光素 是美国AAT Bioquest生产的荧光素酶,该酶是由来自北美萤火虫(Photinus pyralis)的克隆基因表达的荧光素酶,其可以使用生物发光试剂检测ATP或荧光素底物进行研究或生产试剂盒。 这种重组酶有可能消除从天然来源纯化的荧光素酶中发现的季节性和区域性变异性。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的荧光素酶 重组荧光素 。 

百萤问号:荧光素酶到底该如何检测?

图1.在带有NOVOstar酶标仪(BMG Labtech)的白色96孔板中,使用Amplite 荧光素酶报告基因基因检测试剂盒检测了荧光素酶活性。 孵育20分钟后,仍可检测到荧光素酶活性为0.1 pg /孔。

 

 

参考文献

Measuring HIV Neutralization in a Luciferase Reporter Gene Assay
Authors: Montefiori DC.
Journal: Methods Mol Biol (2009): 395

A cell-based luciferase assay amenable to high-throughput screening of inhibitors of arenavirus budding
Authors: Capul AA, de la Torre JC.
Journal: Virology (2008): 107

An optimized luciferase bioluminescent assay for coenzyme A
Authors: Marques SM, da Silva JC.
Journal: Anal Bioanal Chem (2008): 2161

Application of the dual-luciferase reporter assay to the analysis of promoter activity in Zebrafish embryos
Authors: Alcaraz-Perez F, Mulero V, Cayuela ML.
Journal: BMC Biotechnol (2008): 81

Firefly luciferase complementation imaging assay for protein-protein interactions in plants
Authors: Chen H, Zou Y, Shang Y, Lin H, Wang Y, Cai R, Tang X, Zhou JM.
Journal: Plant Physiol (2008): 368

Quantitation of autophagy by luciferase release assay
Authors: Ketteler R, Seed B.
Journal: Autophagy (2008): 801

Quantitative assessment of the knockdown efficiency of morpholino antisense oligonucleotides in zebrafish embryos using a luciferase assay
Authors: Kamachi Y, Okuda Y, Kondoh H.
Journal: Genesis (2008): 1

Determination of silencing potency of synthetic and RNase III-generated siRNA using a secreted luciferase assay
Authors: Morlighem JE, Petit C, Tzertzinis G.
Journal: Biotechniques (2007): 599

A luciferase-based budding assay for Ebola virus
Authors: McCarthy SE, Licata JM, Harty RN.
Journal: J Virol Methods (2006): 115

Luciferase reporter assay
Authors: Wettey FR, Jackson AP.
Journal: Subcell Biochem (2006): 423



说明书
荧光素酶 重组荧光素 .pdf