核紫 DCS1 死细胞染料

产品基本信息

产品名称：核紫 DCS1 死细胞染料

储存条件：-20℃干燥

 

产品物理化学光谱特性

溶剂：DMSO

激发波长(nm)：371

发射波长(nm)：454

 

产品介绍

我们的Nuclear Violet DCS1是一种非荧光，DNA选择性和细胞不透性的紫色荧光染料，用于分析死细胞中的DNA含量。 核紫色DCS1在与双链DNA结合后具有明显增强的荧光。 它可用于荧光成像，微孔板和流式细胞仪应用。 这种DNA结合染料可用于死细胞的多色分析。

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染色细胞分析方案

注意：以下方案适用于大多数细胞类型。 生长培养基，细胞密度，其他细胞类型和因子的存在可能影响染色。 玻璃器皿上的残留洗涤剂也可能影响许多生物的染色，并导致在有或没有细胞存在的溶液中出现明亮染色的物质。

 

工作溶液配制

使用缓冲液将核紫 DCS1储备溶液（5mM）稀释到0.5-5μm的浓度。

 

操作步骤

1.将Nuclear Violet DCS1工作溶液加到固定、死亡或凋亡的细胞（悬浮液或粘附细胞）中，并将细胞染色15至60分钟。 在初的实验中，好尝试几种染料浓度来确定产生所需结果的佳浓度。 高染料浓度倾向于引起其他细胞结构的非特异性染色。

2.用荧光显微镜、荧光酶标仪或流式细胞仪直接检测染色细胞。

 

图示

图1.在96孔板上染色的固定和活（非固定）Hela细胞，与核蓝 DCS1 1μm孵育20分钟并在DAPI通道成像。

 

参考文献

Assessment of laser-induced thermal damage in fresh skin with ex vivo confocal microscopy.
Authors: Ortner, Vinzent Kevin and Sahu, Aditi and Haedersdal, Merete and Rajadhyaksha, Milind and Rossi, Anthony Mario
Journal: Journal of the American Academy of Dermatology (2021): e19-e21

Development of a novel flow cytometry-based approach for reticulocytes micronucleus test in rat peripheral blood.
Authors: Chen, Yiyi and Huo, Jiao and Liu, Yunjie and Zeng, Zhu and Zhu, Xuejiao and Chen, Xuxi and Wu, Rui and Zhang, Lishi and Chen, Jinyao
Journal: Journal of applied toxicology : JAT (2021): 595-606

Exploring the utility of Deep Red Anthraquinone 5 for digital staining of ex vivo confocal micrographs of optically sectioned skin.
Authors: Ortner, Vinzent Kevin and Sahu, Aditi and Cordova, Miguel and Kose, Kivanc and Aleissa, Saud and Alessi-Fox, Christi and Haedersdal, Merete and Rajadhyaksha, Milind and Rossi, Anthony Mario
Journal: Journal of biophotonics (2021): e202000207

A novel method to purify neutrophils enables functional analysis of zebrafish hematopoiesis.
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Journal: Genes to cells : devoted to molecular & cellular mechanisms (2020): 770-781

Analysis of erythroid maturation in the nonlysed bone marrow with help of radar plots facilitates detection of flow cytometric aberrations in myelodysplastic syndromes.
Authors: Violidaki, Despoina and Axler, Olof and Jafari, Katayoon and Bild, Filippa and Nilsson, Lars and Mazur, Joanna and Ehinger, Mats and Porwit, Anna
Journal: Cytometry. Part B, Clinical cytometry (2020): 399-411

Identification of Cancer-Associated Circulating Cells in Anal Cancer Patients.
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Journal: Cancers (2020)

Influence of Polymer Charge on the Localization and Dark- and Photo-Induced Toxicity of a Potential Type I Photosensitizer in Cancer Cell Models.
Authors: Lindgren, Mikael and Gederaas, Odrun A and Siksjø, Monica and Hansen, Tom A and Chen, Lena and Mettra, Bastien and Andraud, Chantal and Monnereau, Cyrille
Journal: Molecules (Basel, Switzerland) (2020)

TEM observation of compacted DNA of Synechococcus elongatus PCC 7942 using DRAQ5 labelling with DAB photooxidation and osmium black.
Authors: Ghosh, Ilika and Atsuzawa, Kimie and Arai, Aoi and Ohmukai, Ryuzo and Kaneko, Yasuko
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[The Establishment of a Three-color Flow Cytometry Approach for the Scoring of Micronucleated Reticulocytes in Rat Bone Marrow].
Authors: Zeng, Zhu and Zhu, Xue-Jiao and Huo, Jiao and Liu, Yun-Jie and Peng, Zi-Hao and Chen, Jin-Yao and Zhang, Li-Shi
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Automated Triage Radiation Biodosimetry: Integrating Imaging Flow Cytometry with High-Throughput Robotics to Perform the Cytokinesis-Block Micronucleus Assay.
Authors: Wang, Qi and Rodrigues, Matthew A and Repin, Mikhail and Pampou, Sergey and Beaton-Green, Lindsay A and Perrier, Jay and Garty, Guy and Brenner, David J and Turner, Helen C and Wilkins, Ruth C
Journal: Radiation research (2019): 342-351

 

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