英文flag致电:029-68064558
搜索

细胞培养检测 Pluronic F-127 20% DMSO溶液 CAS 9003-11-6

英文名称:Pluronic® F-127 *20% solution in DMSO*
产品参数
Ex (nm)-Em (nm)-
分子量~13000溶剂Water
存储条件室温储存不要冷冻
产品概述

产品基本信息

产品名称:Pluronic F-127 20% DMSO溶液

CAS:9003-11-6

储存条件:室内储存不要冷冻

保质期:6个月

 

产品物理化学光谱特性

分子量:~12500

溶剂:DMSO

注意:不要冷藏或冻结Pluronic®F-127溶液,因为它可能沉淀。如果观察到沉淀,则可在使用前加热至37°C并进行涡流处理。

 

产品介绍

Pluronic F-127是一种非离子表面活性剂,100%活性,对细胞相对,常用于染料AM酯,如Indo-1 AM,Fura-2 AM,Calcein AM,Fluo-3 AM,Fluo-4 AM,Quest Fluo-8 AM和Quest Rhod-4 AM等,以其水溶性。 Pluronic F-127也可用于分散其他亲脂性探针。应进行适当的控制以确保Pluronic F-127不会改变细胞的膜特性。为方便起见,我们还提供10%Pluronic F-127水溶液(Cat#20053)和固体(Cat#20050)。

实验方案

操作说明

注意:以下是我们推荐的方案。该方案仅提供指南,应根据您的具体需求进行修改。

1.使用前立即将等体积的20%Pluronic F-127 DMSO溶液与AM酯DMSO储备溶液(1-5 mM)混合。

2.然后将AM酯和Pluronic F-127的溶液稀释到细胞上样缓冲液如Hanks和20mM Hepes缓冲液(HHBS)中,以达到1μM至10μM的最终AM酯浓度。

注意1:或者,可将稀释度为1:1000至1:500的20%Pluronic F-127储备液稀释至细胞上样缓冲液中,先沉淀0.02至0.04%的工作溶液,然后加入AM酯DMSO储备液实现最终AM酯浓度在1μM和10μM之间的溶液。

注意2:Pluronic F-127的最终浓度通常保持在0.08%或以下。

3.将细胞在所需温度下孵育10分钟至1小时。

注意:通常,希望使用所需的最少量的AM酯来获得足够的荧光信号噪声水平。

4.标记后,在开始实验之前用HHBS或新鲜培养基洗涤细胞。

 

参考文献

3D printed lattices as an activation and expansion platform for T cell therapy
Authors: Bahman Delalat, Frances Harding, Batjargal Gundsambuu, Elena M De-Juan-Pardo, Felix M Wunner, Marie-Luise Wille, Marek Jasieniak, Kristen AL Malatesta, Hans J Griesser, Antonio Simula
Journal: Biomaterials (2017)

GD2-specific CAR T Cells Undergo Potent Activation and Deletion Following Antigen Encounter but can be Protected From Activation-induced Cell Death by PD-1 Blockade
Authors: Tessa Gargett, Wenbo Yu, Gianpietro Dotti, Eric S Yvon, Susan N Christo, John D Hayball, Ian D Lewis, Malcolm K Brenner, Michael P Brown
Journal: Molecular Therapy (2016)

Inflammasome components ASC and AIM2 modulate the acute phase of biomaterial implant-induced foreign body responses
Authors: Susan N Christo, Kerrilyn R Diener, Jim Manavis, Michele A Grimbaldeston, Akash Bachhuka, Krasimir Vasilev, John D Hayball
Journal: Scientific reports (2016)

Hybrid core/shell microparticles and their use for understanding biological processes
Authors: Akash Bachhuka, Susan N Christo, Alex Cavallaro, Kerrilyn R Diener, Agnieszka Mierczynska, Louise E Smith, Romeo Marian, Jim Manavis, John D Hayball, Krasimir Vasilev
Journal: Journal of colloid and interface science (2015): 9--17

Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus
Authors: Susan N Christo, Kerrilyn R Diener, Robert E Nordon, Michael P Brown, Hans J Griesser, Krasimir Vasilev, Farid C Christo, John D Hayball
Journal: Scientific reports (2015): 7760

Individual and population quantitative analyses of calcium flux in T-cells activated on functionalized material surfaces
Authors: Susan N Christo, Ghafar T Sarvestani, Stefani S Griesser, Bryan R Coad, Hans J Griesser, Krasimir Vasilev, Michael P Brown, Kerrilyn R Diener, John D Hayball
Journal: Australian Journal of Chemistry (2012): 45--49

Solid-state capture and real-time analysis of individual T cell activation via self-assembly of binding multimeric proteins on functionalized materials surfaces
Authors: Kerrilyn R Diener, Susan N Christo, Stefani S Griesser, Ghafar T Sarvestani, Krasimir Vasilev, Hans J Griesser, John D Hayball
Journal: Acta biomaterialia (2012): 99--107

 

相关产品

产品名称 货号
细胞培养检测 Pluronic F-127 Cat#20050
细胞培养检测 Pluronic F-127 10%水溶液 Cat#20053