细胞培养检测 Pluronic F-127
货号20050存储条件RT
规格10 g价格900
Ex (nm)Em (nm)
分子量~13000溶剂Water
产品详细介绍


简要概述

产品基本信息

货号:20050

产品名称:Pluronic F-127 细胞培养检测

规格:10g

储存条件:室内储存不要冷冻

保质期:12个月

 

产品物理化学光谱特性

分子量:~12500

溶剂:DMSO

 

产品介绍

        Pluronic F-127是一种非离子表面活性剂,100%活性,对低浓度细胞无毒,常用于染料AM酯,如Indo-1 AM,Fura-2 AM,Calcein AM,Fluo-3 AM,Fluo-4 AM,Quest Fluo-8 AM和Quest Rhod-4 AM等,以改善其水溶性。 Pluronic F-127也可用于分散其他亲脂性探针。应进行适当的控制以确保Pluronic F-127不会改变细胞的膜特性。为方便起见,我们还提供20%Pluronic F-127 DMSO溶液(Cat#20052)和10%Pluronic F-127水溶液(Cat#20053)。



产品说明书

操作说明

注意:以下是我们推荐的方案。该方案仅提供指南,实际应根据您的具体需求进行修改。

1.将1克Pluronic F-127溶于10毫升蒸馏水中,制成10%(w / v)原液,或2克Pluronic F-127在10毫升无水二甲基亚砜(DMSO)中制成20%(w / v)储备溶液。这些可能需要在40至50℃的温度下加热约30分钟。在室温下储存溶液。

注意:不要冷却或冷冻Pluronic F-127溶液,因为它可能会沉淀。如果观察到沉淀,可以通过加热至37℃使沉淀物溶解并在使用前涡旋。

2.将10%或20%Pluronic®F-127储备液(来自步骤1)稀释到细胞上样缓冲液中,如Hanks和20 mM Hepes缓冲液(HHBS),稀释度为1:1000至1:500,以达到0.02至0.04%的工作溶液。

3.然后将AM酯的DMSO储备溶液稀释到0.02至0.04%的工作溶液(来自步骤2)中,以获得1μM至10μM的最终AM酯浓度。

注意:Pluronic F-127的最终浓度通常保持在0.08%或以下。

4.将细胞在所需温度下孵育10分钟至1小时。

注意:通常,希望使用所需的最少量的AM酯来获得足够的荧光信号噪声水平。

5.标记后,在开始实验之前用HHBS或新鲜培养基洗涤细胞。

 

参考文献

Engineering Human Ventricular Heart Tissue Based on Macroporous Iron Oxide Scaffolds
Authors: Hui Yang, Lai Wei, Chen Liu, Weiyi Zhong, Bin Li, Yuncan Chen, Rui Han, Jiexian Zhuang, Jianxun Qu, Hongyue Tao
Journal: Acta biomaterialia (2019)

3D printed lattices as an activation and expansion platform for T cell therapy
Authors: Bahman Delalat, Frances Harding, Batjargal Gundsambuu, Elena M De-Juan-Pardo, Felix M Wunner, Marie-Luise Wille, Marek Jasieniak, Kristen AL Malatesta, Hans J Griesser, Antonio Simula
Journal: Biomaterials (2017)

GD2-specific CAR T Cells Undergo Potent Activation and Deletion Following Antigen Encounter but can be Protected From Activation-induced Cell Death by PD-1 Blockade
Authors: Tessa Gargett, Wenbo Yu, Gianpietro Dotti, Eric S Yvon, Susan N Christo, John D Hayball, Ian D Lewis, Malcolm K Brenner, Michael P Brown
Journal: Molecular Therapy (2016)

Inflammasome components ASC and AIM2 modulate the acute phase of biomaterial implant-induced foreign body responses
Authors: Susan N Christo, Kerrilyn R Diener, Jim Manavis, Michele A Grimbaldeston, Akash Bachhuka, Krasimir Vasilev, John D Hayball
Journal: Scientific reports (2016)

Hybrid core/shell microparticles and their use for understanding biological processes
Authors: Akash Bachhuka, Susan N Christo, Alex Cavallaro, Kerrilyn R Diener, Agnieszka Mierczynska, Louise E Smith, Romeo Marian, Jim Manavis, John D Hayball, Krasimir Vasilev
Journal: Journal of colloid and interface science (2015): 9--17

Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus
Authors: Susan N Christo, Kerrilyn R Diener, Robert E Nordon, Michael P Brown, Hans J Griesser, Krasimir Vasilev, Farid C Christo, John D Hayball
Journal: Scientific reports (2015): 7760

Individual and population quantitative analyses of calcium flux in T-cells activated on functionalized material surfaces
Authors: Susan N Christo, Ghafar T Sarvestani, Stefani S Griesser, Bryan R Coad, Hans J Griesser, Krasimir Vasilev, Michael P Brown, Kerrilyn R Diener, John D Hayball
Journal: Australian Journal of Chemistry (2012): 45--49

Solid-state capture and real-time analysis of individual T cell activation via self-assembly of binding multimeric proteins on functionalized materials surfaces
Authors: Kerrilyn R Diener, Susan N Christo, Stefani S Griesser, Ghafar T Sarvestani, Krasimir Vasilev, Hans J Griesser, John D Hayball
Journal: Acta biomaterialia (2012): 99--107

 

相关产品

产品名称 货号
细胞培养检测 Pluronic F-127 20% DMSO溶液 Cat#20052
细胞培养检测 Pluronic F-127 10%水溶液 Cat#20053


说明书
细胞培养检测 Pluronic F-127 .pdf