Portelite 荧光法RNA定量试剂盒*优化于 Cytocite™ 和 Qubit™ 荧光仪*

货号17658存储条件 Multiple
规格100 Tests价格1176
Ex (nm)509Em (nm)529
分子量溶剂
产品详细介绍


简要概述

产品基本信息

货号:17658

产品名称:Portelite 荧光法RNA定量试剂盒

规格:100 Tests

储存条件:保存在冰箱-15℃干燥

保质期:12个月

  

成分

成分A:StrandBrite 绿色 0.25 mL(在DMSO中为200X)
成分B:测定缓冲液 1瓶(50ml)
成分C:RNA标准#1 1 mL(RNA:0 ng / µL)
成分D:RNA标准#2 4 X 250 µL(RNA:10 ng / µL)

 

适用仪器


Qubit 荧光计  
激发: 480nm
发射: 530nm
器材: 0.2 mL PCR小瓶
CytoCite 荧光计  
激发: 480nm
发射: 530nm
推荐孔板: 0.2 mL PCR小瓶

 

产品介绍

检测和定量少量RNA对于多种生物学应用极为重要,例如在进行Northern blot分析,S1核酸酶测定,RNase保护测定,cDNA文库制备,反转录之前,测量体外转录RNA的产量和测定RNA浓度。 PCR和差异显示PCR。测量核酸浓度最常用的技术是测定260 nm的吸光度。基于吸收率的方法受到蛋白质,游离核苷酸和其他紫外线吸收化合物引起的干扰的限制。敏感和选择性荧光核酸染色剂的使用减轻了这种干扰问题。StrandBrite RNA定量试剂是一种超灵敏的荧光核酸染料,用于定量溶液中的RNA。使用CytoCite 或Qubit 荧光仪,StrandBrite RNA定量试剂可检测低至5 ng / mL的RNA。我们的StrandBrite Green荧光RNA定量试剂盒包括我们的StrandBrite Green核酸染色剂,稳定性极高。它提供了一种定量溶液中RNA的简便方法。

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参考文献

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说明书
Portelite 荧光法RNA定量试剂盒*优化于 Cytocite™ 和 Qubit™ 荧光仪*.pdf