细胞增殖WST-8检测试剂 50mM水溶液 CAS 193149-74-5
英文名称：ReadiUse™ WST-8 *50 mM aqueous solution*
产品参数
| Ex (nm) | - | Em (nm) | - |
| 分子量 | 600.5 | 溶剂 | Water |
| 存储条件 | 避免光照, 在零下15度以下保存 |
产品概述
产品货期
咨询
产品优势
1.灵敏度高
2.稳定性好
适用范围
用于检测细胞代谢活性
产品介绍
WST-8（2-(2-甲氧基-4-硝基苯基)-3-(4-硝基苯基)-5-(2,4-二磺酸苯基)-2H-四唑单钠盐）是一种水溶性四唑盐，用于检测细胞代谢活性。
在中性pH条件下，存在中间电子受体1-甲氧基吩嗪硫酸甲酯（1-methoxy PMS）时，依赖NADPH的细胞氧化还原酶通过质膜电子传递系统，能将细胞外无色的WST-8（细胞不可渗透）还原为水溶性橙色甲臜染料，其最大吸收波长为460 nm。
WST-8具有高的稳定性，且由于细胞毒性极低，可耐受长时间孵育（如24至48小时）。相比其他四唑盐（如MTT、XTT或MTS），WST-8在细胞增殖和毒性检测中能更灵敏地通过比色法测定活细胞数量，具有更高的检测灵敏度。该试剂通常用作细胞增殖实验中的细胞活性指示剂。
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