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刃天青钠盐 细胞增殖检测 CAS 62758-13-8

英文名称:Resazurin, sodium salt *CAS 62758-13-8*
产品参数
Ex (nm)571Em (nm)584
分子量251.17溶剂DMSO
存储条件在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:刃天青钠盐 细胞增殖检测

CAS:62758-13-8

储存条件:-15℃避光防潮

保质期:12个月

 

产品物理化学光谱特性

分子量:251.17

溶剂:DMSO

激发波长(nm):571

发射波长(nm):585

 

产品介绍

刃天青钠盐 细胞增殖检测 是美国AAT Bioquest生产的用于检测细胞增殖的试剂,刃天青是一种,水溶性,易氧化还原的染料,在许多生物过程中在试卤灵还原条件下,它从蓝色/无色荧光状态到粉红色/明亮荧光之间改变。刃天青加入到活细胞中,很快被还原,其作用机制是通过细胞中的酶催化还是化学反应,至今还不清楚。这种特性使它广泛地用于细胞增值,细胞活力和细胞毒性的检测。刃天青可以在培养期间的任何时间加入。为了检测细胞增值,一般建议在对数生长期加入。培育时间的选择可能依赖于通过细胞系检测所得到的代谢速率。它所产生的荧光可以用544 nm/590 nm滤光器读取,吸光度可以通过分光光度计在570nm处测定。百萤生物是AAT Bioquest的中国代理商,为您提供优质的刃天青钠盐 细胞增殖检测 。 

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参考文献

A fluorometric study of relative ocular lens cytosensitivity to multipurpose contact lens solutions using the resazurin assay method
Authors: Oriowo MO.
Journal: Toxicol In Vitro (2006): 1548

A resazurin-based biosensor for organic pollutants
Authors: Tizzard AC, Bergsma JH, Lloyd-Jones G.
Journal: Biosens Bioelectron (2006): 759

Application of the resazurin microtitre assay for detection of multidrug resistance in Mycobacterium tuberculosis in Algiers
Authors: Nateche F, Martin A, Baraka S, Palomino JC, Khaled S, Portaels F.
Journal: J Med Microbiol (2006): 857

Determination of the diagnostic value of the resazurin reduction assay for evaluating boar semen by receiver operating characteristic analysis
Authors: Zrimsek P, Kosec M, Kunc J, Mrkun J.
Journal: Asian J Androl (2006): 343

Development and application of a resazurin-based biomass activity test for activated sludge plant management
Authors: McNicholl BP, McGrath JW, Quinn JP.
Journal: Water Res. (2006)

Development of resazurin microtiter assay for drug sensibility testing of Trypanosoma cruzi epimastigotes
Authors: Rolon M, Vega C, Escario JA, Gomez-Barrio A.
Journal: Parasitol Res (2006): 103

Evaluation of the resazurin microtiter assay for rapid detection of ofloxacin resistance in M. tuberculosis
Authors: Umubyeyi AN, Martin A, Zissis G, Struelens M, Karita E, Portaels F.
Journal: Int J Tuberc Lung Dis (2006): 808

Rapid susceptibility test for Mycobacterium tuberculosis to isoniazid and rifampin with resazurin method in screw-cap tubes
Authors: Coban AY, Cekic Cihan C, Bilgin K, Uzun M, Akgunes A, Cetinkaya E, Durupinar B.
Journal: J Chemother (2006): 140

Comparative evaluation of the nitrate reduction assay, the MTT test, and the resazurin microtitre assay for drug susceptibility testing of clinical isolates of Mycobacterium tuberculosis
Authors: Montoro E, Lemus D, Echemendia M, Martin A, Portaels F, Palomino JC.
Journal: J Antimicrob Chemother (2005): 500

Detection of mitochondrial respiratory dysfunction in circulating lymphocytes using resazurin
Authors: Abu-Amero KK, Bosley TM.
Journal: Arch Pathol Lab Med (2005): 1295