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Screen Quest 10X细胞染色缓冲液 *含酚红*

英文名称:Screen Quest™ 10X cell staining buffer with Phenol Red Plus™
产品参数
Ex (nm)-Em (nm)-
分子量N/A溶剂-
存储条件在零下15度以下保存, 避免光照
产品概述

产品基本信息

产品名称:Screen Quest 10X细胞染色缓冲液

储存条件:-15℃避光防潮

保质期:24个月

 

产品介绍

即用型缓冲液,针对荧光细胞成像进行了优化。 在某些情况下,此缓冲液可明显增强成像信号。百萤生物是AAT Bioquest的中国代理商,为您提供优质的Screen Quest 10X细胞染色缓冲液。 

 

图示

图1.带有Phenol Red Plus 的Screen Quest 10X细胞染色缓冲液的图

图2.使用Cal-520 AM在CHO-M1细胞中测量了ATP剂量反应。 将CHO-M1细胞以50,000个细胞/ 100 µL /孔在黑色96孔板中过夜接种。 加入含1X Phenol Red Plus 细胞染色缓冲液的HH缓冲液中的100 µL 10ug / ml Cal-520 AM,并在37oC下孵育60分钟。 加入ATP(50µL /孔)以达到指示的浓度。

 

参考文献

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Flow cytometric kinetic assay of calcium mobilization in whole blood platelets using Fluo-3 and CD41
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A simple numerical model of calcium spark formation and detection in cardiac myocytes
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Monitoring calcium in outer hair cells with confocal microscopy and fluorescence ratios of fluo-3 and fura-red
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Journal: Shi Yan Sheng Wu Xue Bao (1998): 323

Loading and localization of Fluo-3 and Fluo-3/AM calcium indicators in sinapis alba root tissue
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Journal: Folia Histochem Cytobiol (1997): 41

Nucleoplasmic and cytoplasmic differences in the fluorescence properties of the calcium indicator Fluo-3
Authors: Perez-Terzic C, Stehno-Bittel L, Clapham DE.
Journal: Cell Calcium (1997): 275

Detection of a trigger zone of bradykinin-induced fast calcium waves in PC12 neurites
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Journal: Pflugers Arch (1996): 893

Improved four-color flow cytometry method using fluo-3 and triple immunofluorescence for analysis of intracellular calcium ion ([Ca2+]i) fluxes among mouse lymph node B- and T-lymphocyte subsets
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