Screen Quest 10X细胞染色缓冲液 *含酚红*

货号36300存储条件 在零下15度以下保存, 避免光照
规格10 mL价格1272
Ex (nm)Em (nm)
分子量溶剂
产品详细介绍


简要概述

产品基本信息

货号:36300

产品名称:Screen Quest 10X细胞染色缓冲液

规格:10ml

储存条件:-15℃避光防潮

保质期:24个月

 

产品介绍

即用型缓冲液,针对荧光细胞成像进行了优化。 在某些情况下,此缓冲液可明显增强成像信号。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的Screen Quest 10X细胞染色缓冲液。 

 

图示

图1.带有Phenol Red Plus 的Screen Quest 10X细胞染色缓冲液的图

图2.使用Cal-520 AM在CHO-M1细胞中测量了ATP剂量反应。 将CHO-M1细胞以50,000个细胞/ 100 µL /孔在黑色96孔板中过夜接种。 加入含1X Phenol Red Plus 细胞染色缓冲液的HH缓冲液中的100 µL 10ug / ml Cal-520 AM,并在37oC下孵育60分钟。 加入ATP(50µL /孔)以达到最终指示的浓度。

 

参考文献

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Novel fluo-4 analogs for fluorescent calcium measurements
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Amplitude distribution of calcium sparks in confocal images: theory and studies with an automatic detection method
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Journal: Biophys J (1999): 606

Flow cytometric kinetic assay of calcium mobilization in whole blood platelets using Fluo-3 and CD41
Authors: do Ceu Monteiro M, Sansonetty F, Goncalves MJ, O'Connor JE.
Journal: Cytometry (1999): 302

A simple numerical model of calcium spark formation and detection in cardiac myocytes
Authors: Smith GD, Keizer JE, Stern MD, Lederer WJ, Cheng H.
Journal: Biophys J (1998): 15

Monitoring calcium in outer hair cells with confocal microscopy and fluorescence ratios of fluo-3 and fura-red
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Journal: Shi Yan Sheng Wu Xue Bao (1998): 323

Loading and localization of Fluo-3 and Fluo-3/AM calcium indicators in sinapis alba root tissue
Authors: Tretyn A, Kado RT, Kendrick RE.
Journal: Folia Histochem Cytobiol (1997): 41

Nucleoplasmic and cytoplasmic differences in the fluorescence properties of the calcium indicator Fluo-3
Authors: Perez-Terzic C, Stehno-Bittel L, Clapham DE.
Journal: Cell Calcium (1997): 275

Detection of a trigger zone of bradykinin-induced fast calcium waves in PC12 neurites
Authors: Reber BF, Schindelholz B.
Journal: Pflugers Arch (1996): 893

Improved four-color flow cytometry method using fluo-3 and triple immunofluorescence for analysis of intracellular calcium ion ([Ca2+]i) fluxes among mouse lymph node B- and T-lymphocyte subsets
Authors: Greimers R, Trebak M, Moutschen M, Jacobs N, Boniver J.
Journal: Cytometry (1996): 205



说明书
Screen Quest 10X细胞染色缓冲液 *含酚红*.pdf