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膜电位荧光探针TMA-DPH CAS 115534-33-3

英文名称:TMA-DPH *CAS#: 115534-33-3*
产品参数
Ex (nm)-Em (nm)-
分子量461.62溶剂DMSO
存储条件在零下15度以下保存, 避免光照
产品概述

膜电位荧光探针TMA-DPH是美国AAT Bioquest生产的用于检测膜电位的荧光探针,TMA-DPH是用于膜流动性测量的荧光探针。它在脂蛋白和类似类似系统的单层动力学研究中有用。DPH及其衍生物(例如TMA-DPH)是圆柱形的并且具有荧光发射转变偶极子,其基本上与它们的长分子轴平行排列。因此,它们对由与周围脂质的相互作用引起的重新定向非常敏感。它们广泛用于旋转运动的荧光偏振研究。百萤生物是AAT Bioquest的中国代理商,为您提供优质的膜电位荧光探针TMA-DPH。 

实验方案

操作步骤

        以下程序适用于大多数细胞类型。生长培养基,细胞密度,其他细胞类型的存在和其他因素可能影响染色。玻璃器皿上的残留洗涤剂也可能影响许多生物的染色,导致在有或没有细胞存在的溶液中出现明亮染色的物质。


1)通过离心沉淀细胞,并将细胞重悬于缓冲盐溶液或培养基中,在pH7.4下具有佳染料结合。培养物中的贴壁细胞可以在盖玻片上或细胞培养孔中原位染色。
2)制备10-50mM的TMA-DPH DMSO储备溶液。
3)在Hanks和20mM Hepes缓冲液pH 7.4中使用0.5和5μM之间的浓度添加TMA-DPH染色,并在37℃,5%CO2培养箱中孵育5分钟。在初的实验中,好在整个建议范围内尝试几种染料浓度,以确定产生佳染色的浓度。
4)洗涤细胞并重悬于适当的pH 7.4的Hepes缓冲液中。

 

参考文献

Comparison of cytotoxicity and membrane efflux pump inhibition in HepG2 cells induced by single-walled carbon nanotubes with different length and functional groups
Authors: Zhuoyan Shen, Jialu Wu, Yue Yu, Su Liu, Wei Jiang, Habiba Nurmamat, Bing Wu
Journal: Scientific Reports (2019): 7557

Size-dependent effects of polystyrene microplastics on cytotoxicity and efflux pump inhibition in human Caco-2 cells
Authors: Bing Wu, Xiaomei Wu, Su Liu, Zhizhi Wang, Ling Chen
Journal: Chemosphere (2019)

Cytotoxicity and efflux pump inhibition induced by molybdenum disulfide and boron nitride nanomaterials with sheet-like structure
Authors: Su Liu, Zhuoyan Shen, Bing Wu, Yue Yu, Hui Hou, Xu-Xiang Zhang, Hong-qiang Ren
Journal: Environmental Science & Technology (2017)

Interaction of Hsp90 with phospholipid model membranes
Authors: Muhan Zhang, Daoying Wang, Pengpeng Li, Chong Sun, Rong Xu, Zhiming Geng, Weimin Xu, Zhaoqi Dai
Journal: Biochimica et Biophysica Acta (BBA)-Biomembranes (2017)

Comparison of Cytotoxicity and Inhibition of Membrane ABC Transporters Induced by MWCNTs with Different Length and Functional Groups
Authors: Jing Yu, Su Liu, Bing Wu, Zhuoyan Shen, Gary N Cherr, Xu-Xiang Zhang, Mei Li
Journal: Environmental science & technology (2016): 3985--3994

Low levels of graphene and graphene oxide inhibit cellular xenobiotic defense system mediated by efflux transporters
Authors: Su Liu, Wei Jiang, Bing Wu, Jing Yu, Haiyan Yu, Xu-Xiang Zhang, Cristina Torres-Duarte, Gary N Cherr
Journal: Nanotoxicology (2016): 597--606