COVID-19荧光底物Z-KAGG-AMC

产品基本信息

产品名称：COVID-19荧光底物Z-KAGG-AMC

储存条件：-15℃避光防潮

保质期：12个月

产品物理化学光谱特性

外观：固体

溶剂：DMSO

激发波长(nm)：341

发射波长(nm)：441

产品介绍

冠状病毒（CoV）是一种可以感染人类和多种动物，因其形态看上去像中世纪欧洲帝的皇冠，因此命名为“冠状病毒”。这类病毒具有胃肠道、呼吸道和神经系统的嗜性，可以导致多种疾病。在2019年底，一种新型的冠状病毒被称为严重急性呼吸系统综合症冠状病毒2（SARS-CoV-2），开始在全球蔓延。即使大多数受感染的患者仅患有轻度症状，例如发烧和咳嗽，但该疾病可能发展为致命的肺炎和急性呼吸衰竭病例，尤其是在多病体弱的的老人中。该病毒仅仅从出现开始的三四个月，全球已经感染了超过100万人。当前，尚无用于Covid-19的任何具体有效的选择。目前，Covid-19的临床主要是对症，并结合上市的抗病毒（如瑞德昔韦和）以继发感染。目前全都迫切需要开发抗SARS-CoV-2的特异性抗病毒方法和疫苗，而冠状病毒主蛋白酶是抗CoV设计的重要靶点，在病毒基因表达和复制中起着关键作用。目前已掌握的人类免疫缺陷病毒（HIV）和丙型肝炎的成功策略是抑制蛋白水解酶消灭病毒蛋白酶。而且证明了蛋白酶抑制剂病毒感染具有非常大的潜力。同样，SARS-CoV-2病毒必须要进行复制的酶便是蛋白酶，因此Covid-19是抗病毒极好的靶点。

冠状病毒是一种单链RNA正链包膜B型冠状病毒。像其他两种导致严重急性呼吸系统综合症（SARS）和中东呼吸系统综合症（MERS）的冠状病毒一样，SARS-CoV-2编码由非结构，结构和辅助蛋白组成。非结构蛋白包括3-胰凝乳蛋白酶样蛋白酶（3CLpro），木瓜蛋白酶，解旋酶和RNA依赖性RNA聚合酶（RNA依赖性RNA聚合酶（RdRp）），结构蛋白包括刺突糖蛋白。从大的多蛋白1ab上的11个切割位点开始，从病毒RNA翻译的多蛋白的加工是必不可少的，因此，主要的蛋白酶被认为是预防病毒模仿的诱人靶标。冠状病毒的木瓜蛋白酶样蛋白酶（PLpro）进行非结构蛋白的蛋白水解成熟，这些蛋白在病毒复制中起作用，并执行宿主细胞因子的去泛素化，从而破坏抗病毒反应。Z-KAGG-AMC被木瓜蛋白酶样蛋白酶裂解，得到高度荧光的AMC产物。释放的AMC的荧光强度与蛋白酶活性成正比。Z-KAGG-AMC可用于筛选和研究PLpro抑制剂的动力学。

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参考文献

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Analysis of Coronavirus Temperature-Sensitive Mutants Reveals an Interplay between the Macrodomain and Papain-Like Protease Impacting Replication and Pathogenesis.
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Characterization of the Papain-Like Protease p29 of the Hypovirus CHV1-CN280 in Its Natural Host Fungus Cryphonectria parasitica and Nonhost Fungus Magnaporthe oryzae.
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Journal: Phytopathology (2019): 736-747

First detection of novel enterovirus G recombining a torovirus papain-like protease gene associated with diarrhoea in swine in South Korea.
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Journal: Transboundary and emerging diseases (2019): 1023-1028

Identification and design of novel small molecule inhibitors against MERS-CoV papain-like protease via high-throughput screening and molecular modeling.
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Porcine epidemic diarrhea virus papain-like protease 2 can be noncompetitively inhibited by 6-thioguanine.
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The papain-like protease determines a virulence trait that varies among members of the SARS-coronavirus species.
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MERS-CoV papain-like protease (PLpro): expression, purification, and spectroscopic/thermodynamic characterization.
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