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新型钙离子荧光探针Calbryte-520L AM(停产)

产品参数
Ex (nm)-Em (nm)-
分子量-溶剂-
存储条件-
产品概述

产品基本信息

货号:20640

产品名称:新型钙离子荧光探针Calbryte-520L AM

规格:10x50 ug

储存条件:-15℃避光防潮

保质期:24个月

 

产品物理化学光谱特性

分子量:870.72

溶剂:水

激发波长(nm):492

发射波长(nm):514

 

适用仪器


荧光显微镜  
激发: FITC
发射: FITC
推荐孔板: 黑色透明
荧光酶标仪  
激发: 490nm
发射: 525nm
cutoff: 515nm
推荐孔板: 黑色透明
读取模式: 底读模式

 

产品介绍

新型钙离子荧光探针Calbryte-520L AM是美国AAT Bioquest生产的钙离子荧光探针,细胞内钙通量测定法是监测信号转导途径和G蛋白偶联受体(GPCR)和钙通道靶标的高通量筛选的一种广泛使用的方法。随后在1989年推出Fluo-3,随后开发了具有改善的信噪比的Fluo-4,Fluo-8和Cal-520,并成为共聚焦显微镜,流式细胞术和高通量筛选应用中广泛使用的Ca2 +指示剂。但是,Fluo-4仍然存在一些严重问题。例如,对于Fluo-3,在所有大多数使用Fluo-4 AM进行的细胞内钙测定中,都需要丙磺舒以防止细胞加载的Fluo-4泄漏出细胞。丙磺舒与基于Fluo-4的钙测定法的结合使用会损害测定结果,因为众所周知丙磺舒具有多种复杂的细胞作用。 Calbryte 520L,AM是一种新型的荧光且可渗透细胞的钙指示剂。像其他染料AM细胞加载一样,Calbryte 520L AM酯不发荧光,一旦进入细胞内部,它就会被细胞内酯酶水解并被激活。激活的指示剂是一种极性分子,不再能够自由扩散通过细胞膜,基本上被困在细胞内部。 Calbryte 520L对钙离子的亲和力低,Kd约为100 uM。 Calbryte 520L在高浓度钙离子存在下产生明亮的荧光信号。它具有与Fluo-4相同的激发和发射波长,因此相同的Fluo-4测定设置可轻松应用于基于Calbryte 520L的钙测定。百萤生物是AAT Bioquest的中国代理商,为您提供最优质的新型钙离子荧光探针Calbryte-520L AM。 

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钙离子篇:时间轴式讲解应用于钙离子检测的探针

 

参考文献

Calreticulin regulates TGF-β1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling
Authors: Yanjiao Wu, Xiaoli Xu, Lunkun Ma, Qian Yi, Weichao Sun, Liling Tang
Journal: The International Journal of Biochemistry & Cell Biology (2017)

Dexmedetomidine reduces hypoxia/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neurons
Authors: Jia Liu, Qing Du, He Zhu, Yu Li, Maodong Liu, Shoushui Yu, Shilei Wang
Journal: Int J Clin Exp Med (2017): 6861--6868

Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells
Authors: Jiang Lu, Xue-qin Yao, Xin Luo, Yu Wang, Sookja Kim Chung, He-xin Tang, Chi Wai Cheung, Xian-yu Wang, Chen Meng, Qing Li
Journal: Neural Regeneration Research (2017): 945

Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels
Authors: Gang Yang, Zhenghua Xiao, Xiaomei Ren, Haiyan Long, Kunlong Ma, Hong Qian, Yingqiang Guo
Journal: Scientific Reports (2017): 41781

Di (2-ethylhexyl) phthalate-induced apoptosis in rat INS-1 cells is dependent on activation of endoplasmic reticulum stress and suppression of antioxidant protection
Authors: Xia Sun, Yi Lin, Qiansheng Huang, Junpeng Shi, Ling Qiu, Mei Kang, Yajie Chen, Chao Fang, Ting Ye, Sijun Dong
Journal: Journal of cellular and molecular medicine (2015): 581--594

The effect of mitochondrial calcium uniporter on mitochondrial fission in hippocampus cells ischemia/reperfusion injury
Authors: Lantao Zhao, Shuhong Li, Shilei Wang, Ning Yu, Jia Liu
Journal: Biochemical and biophysical research communications (2015): 537--542

Fungus induces the release of IL-8 in human corneal epithelial cells, via Dectin-1-mediated protein kinase C pathways.
Authors: Xu-Dong Peng, Gui-Qiu Zhao, Jing Lin, Nan Jiang, Qiang Xu, Cheng-Cheng Zhu, Jain-Qiu Qu, Lin Cong, Hui Li
Journal: International journal of ophthalmology (2014): 441--447

Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem/progenitor cells
Authors: Qing Li, Jiang Lu, Xianyu Wang
Journal: Neural regeneration research (2014): 2002

Role of mitochondrial calcium uniporter in regulating mitochondrial fission in the cerebral cortexes of living rats
Authors: Nan Liang, Peng Wang, Shilei Wang, Shuhong Li, Yu Li, Jinying Wang, Min Wang
Journal: Journal of Neural Transmission (2014): 593--600

Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve--mast cell interaction in a hapten-induced mouse model of atopic dermatitis
Authors: M Hagiyama, T Inoue, T Furuno, T Iino, S Itami, M Nakanishi, H Asada, Y Hosokawa, A Ito
Journal: British Journal of Dermatology (2013): 771--778

 

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