DAPI（4',6-二脒基-2-苯基吲哚二盐酸盐）CAS 28718-90-3
英文名称：DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*
产品参数
| Ex (nm) | 359 | Em (nm) | 457 |
| 分子量 | 350.25 | 溶剂 | Water |
| 存储条件 | 在零下15度以下保存, 避免光照 |
产品概述
产品货期
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产品优势
高亲和力结合：特异性识别DNA的A-T富集区
适用范围
用于标记活细胞与固定细胞
产品介绍
DAPI是一种能与DNA强力结合的荧光染料，应用于荧光显微技术。该染料具有细胞膜穿透能力，既可用于固定细胞染色，也可用于活细胞标记。在荧光显微镜下，DAPI需用紫外光激发（与双链DNA结合时最大吸收波长为358nm，发射峰为461nm）。需要注意的是，DAPI的发射光谱较宽，且虽能与RNA结合（此时发射波长红移至500nm左右），但其荧光强度远低于DNA结合状态。得益于独特的蓝色荧光特性（与荧光素、绿色荧光蛋白GFP等绿色荧光分子及Texas Red等红色染料的荧光重叠极少），DAPI非常适用于多色荧光检测实验。除细胞核标记外，该染料还可用于检测细胞培养物中的支原体或病毒DNA污染。
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